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Merck

216275-M

Sigma-Aldrich

5-Carboxyfluorescein Diacetate

Vital stain. Non-fluorescent, hydrophobic agent that permeates intact cell membranes. In the cell, it is enzymatically hydrolyzed to a hydrophilic agent, 5-Carboxyfluorescein that exhibits strong fluorescence.

Synonym(s):

5-Carboxyfluorescein Diacetate, 5-CFDA

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
41116134
NACRES:
NA.51

Pricing and availability is not currently available.

Quality Level

Assay

≥95% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated
protect from light

color

yellow

solubility

DMSO: 5 mg/mL

storage temp.

−20°C

InChI

1S/C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25/h3-11H,1-2H3,(H,28,29)

InChI key

WPUZGNPQMIWOHE-UHFFFAOYSA-N

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This Item
2187921884343209
form

solid

form

powder

form

-

form

solid

solubility

DMSO: 5 mg/mL

solubility

DMSO: soluble

solubility

DMF: soluble, DMSO: soluble, methanol: soluble

solubility

ethanol: soluble

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

assay

≥95% (HPLC)

assay

≥90.0% (HPLC)

assay

≥85% (HPCE)

assay

-

Quality Level

100

Quality Level

-

Quality Level

-

Quality Level

100

General description

Vital stain. Non-fluorescent, hydrophobic agent that permeates intact cell membranes. In the cell, it is enzymatically hydrolyzed to a hydrophilic agent, 5-Carboxyfluorescein that exhibits strong fluorescence.
Vital stain. Non-fluorescent, hydrophobic agent that permeates intact cell membranes. In the cell, it is enzymatically hydrolyzed to a hydrophilic agent, strongly fluorescent 5-Carboxyfluorescein (Cat. No. 216265).

Biochem/physiol Actions

Primary Target
Vital stain

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Bruning, J.W., et al. 1980. J. Immunol. Methods33, 33.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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