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178615

Sigma-Aldrich

Anti-Aquaporin 5 Rabbit pAb

liquid, Calbiochem®

Synonym(s):

Anti-AQP5

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

does not contain

preservative

species reactivity

rat, mouse, human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Anti-Aquaporin 5, rabbit polyclonal, recognizes the ~28 kDa AQP 5 protein in epithelial tissue extracts. It is validated for ELISA, Western blotting, immunofluorescence, and immunohistochemistry.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~28 kDa AQP5 protein.
Recognizes the ~28 kDa AQP 5 protein in epithelial tissue extracts.

Immunogen

a synthetic peptide corresponding to a 17-amino acid sequence in the cytoplasmic region of rat AQP5

Application

ELISA (0.5-1.0 µg/ml)

Immunoblotting (1-10 µg/ml)

Immunofluorescence (2-20 µg/ml)

Immunohistochemistry (2-20 µg/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Analysis Note

Positive Control
Epithelial cells

Other Notes

Does not cross-react with other AQP proteins. The use of 0.5-1.0% milk in all primary/secondary antibody-enzyme conjugate incubations is recommended in order to suppress non-specific bands. Variables associated with assay conditions will dictate the optimal working dilution.
Lee, M.D., et al. 1996. J. Biol. Chem. 271, 8599.
Raina, S., et al. 1995. J. Biol. Chem. 270, 1908.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M Cristina Kenney et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 52(10), 1341-1350 (2004-09-24)
Corneas with edema-related diseases lose transparency, which causes significant vision loss. This study analyzed seven aquaporins (AQPs) in normal corneas, pseudophakic/aphakic bullous keratopathy (PBK/ABK) corneas, Fuchs' dystrophy corneas, keratoconus corneas, post-cataract surgery (PCS) corneas, and normal organ-cultured corneas. RNA levels
Amy P Wong et al.
The Journal of clinical investigation, 119(2), 336-348 (2009-01-24)
The bone marrow compartment is enriched in stem and progenitor cells, and an unidentified subpopulation of these cells can contribute to lung epithelial repair. Here we identify this subpopulation and quantitate its relative contribution to injured airway epithelium. A subpopulation
Mingyu Kwak et al.
Scientific reports, 8(1), 14193-14193 (2018-09-23)
A rare population of salivary gland cells isolated based on c-Kit immunoreactivity are thought to represent tissue stem cells since they exhibit the most robust proliferative and differentiation capacity ex vivo. Despite their high promise for cell-based therapies aimed at
Fernanda de Paula et al.
Journal of anatomy, 238(3), 794-806 (2020-10-21)
Aquaporins (AQPs) are essential to coordinate the transit of water and ions through the cell membrane. In salivary glands (SGs), AQPs have been associated with saliva formation, facilitating water absorption through the epithelium during the formation of hypotonic saliva, which
Ninche Ninche et al.
Development (Cambridge, England), 147(19) (2020-10-01)
Salivary glands exert exocrine secretory function to provide saliva for lubrication and protection of the oral cavity. Its epithelium consists of several differentiated cell types, including acinar, ductal and myoepithelial cells, that are maintained in a lineage-restricted manner during homeostasis

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