Skip to Content
Merck

178488

Sigma-Aldrich

Apoptosis Inhibitor

The Apoptosis Inhibitor, also referenced under CAS 54135-60-3, controls the biological activity of Apoptosis. This small molecule/inhibitor is primarily used for Cancer applications.

Synonym(s):

Apoptosis Inhibitor, M50054, 2,2ʹ-Methylenebis(1,3-cyclohexanedione)

Sign Into View Organizational & Contract Pricing


About This Item

Empirical Formula (Hill Notation):
C13H16O4
CAS Number:
Molecular Weight:
236.26
UNSPSC Code:
12352200
NACRES:
NA.77

Pricing and availability is not currently available.

Quality Level

Assay

≥95% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

color

off-white

solubility

DMSO: 100 mg/mL
ethanol: 20 mg/mL

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C13H16O4/c14-10-3-1-4-11(15)8(10)7-9-12(16)5-2-6-13(9)17/h8-9H,1-7H2

General description

A cell-permeable inhibitor of apoptosis induction (IC50 = 67 µg/ml in FasL-stimulated WC8 cells, 130 µg/ml in etoposide-stimulated U937 cells). Effects attributable to the inhibition of caspase-3 activation but does not directly inhibit caspase-3 even at 1 mg/ml.
A cell-permeable, bis(cyclohexane-1,3-diketone) that is shown to inhibit apoptosis induction in FasL-stimulated WC8 cells (IC50 = 67 µg/ml) and etoposide-stimulated U937 cells (IC50 = 130 µg/ml). The anti-apoptotic effects have been shown to be attributable to inhibition of caspase-3 activation (IC50 = 334 nM), however it does not exhibit any direct inhibition of caspase-3 enzymatic activity. Shown to inhibit the elevation of the liver enzymes, alanine aminotransferase and aspartate aminotransferase, in a murine model of anti-Fas-induced hepatitis. Topical application has been shown to reduce chemotherapy-induced alopecia (hair loss) in newborn rats.

Biochem/physiol Actions

Cell permeable: yes
Primary Target
apoptosis induction
Product does not compete with ATP.
Reversible: no
Target IC50: 67 µg/ml in FasL-stimulated WC8 cells, 130 µg/ml in etoposide-stimulated U937 cells

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Tsuda, T., et al. 2001. Eur. J. Pharmacol.433, 37.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service