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Key Documents

07-164-AF647

Sigma-Aldrich

Anti-phospho H2A.X (Ser139) Antibody, Alexa Fluor™ 647 Conjugate

from rabbit, ALEXA FLUOR™ 647

Synonym(s):

Histone H2A.X, Histone H2A.X, H2a/x

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

conjugate

ALEXA FLUOR™ 647

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

species reactivity (predicted by homology)

vertebrates (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable

isotype

IgG

NCBI accession no.

NP_002096

UniProt accession no.

P16104

shipped in

wet ice

target post-translational modification

phosphorylation (pSer139)

Gene Information

human ... ATP10D(57205) , H2AX(3014)

General description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. H2A.X, also known as H2AFX, is a variant of Histone H2A that is found in a subset of nucleosomes. H2A.X has a unique C-terminal tail that becomes phosphorylated following double-stranded DNA breaks following irradiation or apoptosis. Phosphorylation of H2A.X on Ser139 is one of the earliest markers for apoptosis and DNA damage.

Specificity

Recognizes Histone H2A.X phosphorylated at Serine 139.

Immunogen

Epitope: Phosphorylated Ser139
KLH-conjugated linear peptide corresponding to phospho H2A.X (Ser139).

Application

Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function
Research Sub Category
Histones

Chromatin Biology
See data sheet for cat# 07-164, Anti-phospho H2A.X (Ser139) for additional Western blot, Immnoprecipitation, and ChIP-seq analysis performed using an unconjugated version of this antibody.
This AlexaFluor647 conjugated Anti-phospho Histone H2A.X antibody is validated for use in mmunocytochemistry for the detection of phospho-Histone H2A.X.

Quality

Immunocytochemistry Analysis:

A 1:100 dilution of this antibody detected phospho Histone H2A.X (Ser139) in HeLa cells.
Alexa Fluor™ is a registered trademark of Life Technologies.

Target description

~15 kDa observed

Physical form

Protein A purified
Purified conjugated rabbit polyclonal antibody in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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