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04-300

Sigma-Aldrich

Anti-phospho-Insulin Receptor (Tyr 1322) Antibody, clone 21G12

clone 21g12, Upstate®, from mouse

Synonym(s):

INSR

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

21g12, monoclonal

species reactivity

canine, human, mouse

manufacturer/tradename

Upstate®

technique(s)

ELISA: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

phosphorylation (pTyr1322)

Gene Information

human ... INSR(3643)

Specificity

Recognizes phosphorylated domain of Insulin receptor on Tyrosine 1322.

Immunogen

KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Tyrosine 1322 on phosphorylated insulin receptor

Application

Anti-phospho-Insulin Receptor Antibody, clone 21G12 is an antibody against phospho-Insulin Receptor (Tyr 1322) for use in ELISA & WB.
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling

Quality

Routinely evaluated by immunoblot.

Target description

97 kDa

Physical form

Format: Purified
Lyophilized in 2X PBS containing 0.09% sodium azide, PEG, and sucrose.
Subsequent thiophilic adsorption and size exclusion chromatography

Storage and Stability

2 years at -20°C from date of shipment. Reconstitute prior to use. Reconstitute with 1 mL H2O for 15 min at room temperature. Reconstituted aliquots should be stored frozen at -80°C. Thawed aliquots may be stored at 4°C for up to 3 months. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Includes pervanadate treated HEK293 lysate as a positive control.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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