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Supelco

SUPELCOSIL™ LC-18 (5 µm) HPLC Columns

L × I.D. 2 cm × 4 mm Supelguard Guard Cartridge pkg of 2 ea, Guard Cartridge holder required for use

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501

product name

SUPELCOSIL™ LC-18 Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm

Agency

suitable for USP L1

packaging

pkg of 2 ea

parameter

<70 °C temp. range

technique(s)

HPLC: suitable

L × I.D.

2 cm × 4 mm

matrix

silica gel high purity, spherical

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

120 Å

pH range

2-7.5

application(s)

food and beverages

compatibility

use to protect LC-18, LC-PAH

separation technique

reversed phase

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General description

Guard columns are placed before the analytical column to protect and prevent harmful materials entering into the latter. It also helps as a backup to the in-line filter and also checks for leak through filter. The most suitable are short cartridge which is inserted into the cartridge holder.

Application

SUPELCOSIL™ LC-18 Supelguard Cartridge was used as guard column for Supelcosil™ LC-18 HPLC column to analyze glucose tetrasaccharides in blood and urine samples using HPLC-electrospray ionization mass spectrometry (HPLC-ESI-MS). It was also suitable for analysing polar compounds in non-polar matrices using gas chromatography separation.

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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Haoyue Zhang et al.
Clinical chemistry, 55(3), 527-532 (2009-02-03)
The isotope-labeled intravenous glucose tolerance test (IVGTT) combined with computer modeling is widely used to derive parameters related to glucose metabolism in vivo. Most of these methods involve use of either (2)H(2)-labeled or (13)C(1)-labeled D-glucose as a tracer with GC-MS
J Wu et al.
Journal of chromatography. A, 909(1), 37-52 (2001-02-24)
Polypyrrole (PPY) and poly-N-phenylpyrrole (PPPY) films were prepared and applied for solid-phase microextraction (SPME). The extraction properties of the new films to volatile organic compounds were examined using an SPME device coupled with GC-flame ionization detection. A PPY-coated capillary was
Ike Jeon
Analyzing Food for Nutrition Labeling and Hazardous Contaminants , 95-95 (1994)
L Marai et al.
Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire, 61(8), 840-849 (1983-08-01)
Natural triacylglycerols were resolved by high pressure liquid chromatography on Supelcosil LC-18 columns using a gradient of 30-90% propionitrile in acetonitrile as eluting solvent. The effluent was admitted to a quadrupole mass spectrometer via a direct liquid inlet interface. The
V Stocchi et al.
Analytical biochemistry, 146(1), 118-124 (1985-04-01)
A simple and rapid method for the determination of ATP, ADP, AMP, NADP+, NAD+, NADPH, and NADH in human erythrocytes is described. A single-step extraction procedure employing alkaline medium and CF 50A Amicon ultrafiltration membranes allows a simultaneous and total
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Protocols

Deriviatization, Hydrolysis, and HPLC Analysis of Amino Acids on SUPELCOSIL™ and ASCENTIS® Columns

Amino Acid Analysis is the suitable tool for precise determination of protein quantities but also provides detailed information regarding the relative amino.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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