SAB4700480

Monoclonal Anti-CD45-FITC antibody produced in mouse

clone MEM-28, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-LCA, Anti-PTPRC
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• conjugate: FITC conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: MEM-28, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• technique(s): flow cytometry: suitable
• isotype: IgG1
• NCBI accession no.: NM_002838.3
• UniProt accession no.: P08575
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... PTPRC(5788)

Description

General description

The antibody MEM-28 reacts with all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.

The cluster of differentiation 45 (CD45) gene is mapped to human chromosome 1q31.3-q32.1. It codes for a type I transmembrane protein and is expressed in nucleated hematopoietic cells along within its precursors. The protein consists of a varying extracellular domain and a highly conserved intracellular domain.

Immunogen

Human thymocytes and T lymphocytes

Application

The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 1e6 cells in a suspension. The content of a vial (2 mL) is sufficient for 100 tests.

Biochem/physiol Actions

Cluster of differentiation 45 (CD45) functions as a prototypic receptor and exhibits phosphatase activity. CD45 is implicated in the regulation of T-cell mediated B cell antigen receptor (BCR)-signaling. The encoded protein plays an essential role in antigen-stimulated proliferation of T lymphocytes and in thymic development. CD45 negatively regulates cytokine receptor signaling via suppressing JAK (Janus kinase) activity.

Features and Benefits

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Physical form

Solution in phosphate buffered saline containing 15 mM sodium azide and 0.2% high-grade protease free BSA as a stabilizing agent.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable