S6147
Sephadex® G-100
BioReagent, for molecular biology, DNA grade, medium
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About This Item
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grade
DNA grade
for molecular biology
product line
BioReagent
form
beads
technique(s)
buffer exchange: suitable
matrix active group
phase
swelling
1 g swells to 9-11 mL
bead diameter
40-120 μm
pore size
exclusion limit (25 bp dsDNA; (rA)27)
pH
2-10
application(s)
life science and biopharma
compatibility
Cytiva
foreign activity
DNase, RNase, none detected
storage temp.
room temp
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General description
Sephadex® G-100, Medium is an often-cited gel filtration medium for desalting and buffer exchange of very large molecules. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.
Application
Sephadex® G-100 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify the enzyme polyphenoloxidases isolated from banana by gel filteration.
Features and Benefits
- Excellent recovery
- Minimal sample dilution
- Quick and easy to use
Legal Information
Sephadex is a registered trademark of Cytiva
related product
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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