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R4018

Sigma-Aldrich

Ribonucleic acid, transfer, phenylalanine specific from brewer′s yeast

Synonym(s):

Transfer RNA, Yeast Phe tRNA, tRNA

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About This Item

MDL number:
MFCD00132200
UNSPSC Code:
12352200
NACRES:
NA.55

grade

for molecular biology

form

lyophilized powder

mol wt

25  kDa

solubility

water: 2 mg/mL

storage temp.

2-8°C

General description

Transfer RNA is isolated from brewer′s yeast. This transfer RNA is specific for transfer of the amino acid phenylalanine.

The phenylalanine acceptor activity of 100% pure tRNA is 1,800 pmoles per A260 unit. Comparison of this number to lot-specific analysis may be used as an indication of product purity. Methods to further purify tRNA have been published.

The assay for this product is based on a published method. The activity is measured using a crude preparation of phenylalanyl transfer ribonucleic acid synthetase. The phenylalanine specific tRNA is used as a substrate for the enzyme in the assay.

This product is not suitable for use as carrier tRNA to aid in the ethanol precipitation of very small amounts of DNA. Product Nos. R 8508 and R 5636 are recommended for use as carrier tRNA for DNA purification and precipitation.

Application

Suitable for use:
  • As a tRNA standard
  • As a substrate in tRNA synthatase assays
  • As a substrate in other reactions involving tRNAs

Components

tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.

Preparation Note

Prepared by method of Wimmer, E., et al., Biochemistry, 7, 2623 (1968).

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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W Yan et al.
The EMBO journal, 17(16), 4809-4817 (1998-08-26)
Correct folding of newly synthesized polypeptides is thought to be facilitated by Hsp70 molecular chaperones in conjunction with DnaJ cohort proteins. In Saccharomyces cerevisiae, SSB proteins are ribosome-associated Hsp70s which interact with the newly synthesized nascent polypeptide chain. Here we
Mariana Pavon-Eternod et al.
Nucleic acids research, 37(21), 7268-7280 (2009-09-29)
Increased proliferation and elevated levels of protein synthesis are characteristics of transformed and tumor cells. Though components of the translation machinery are often misregulated in cancers, what role tRNA plays in cancer cells has not been explored. We compare genome-wide
Tissue-specific differences in human transfer RNA expression.
Dittmar, K.A., et al.
PLoS Genetics, 2, 2107-2107 (2006)
Rebecca R Miles et al.
The Journal of biological chemistry, 297(5), 101257-101257 (2021-10-02)
Healing of cutaneous wounds requires the collective migration of epithelial keratinocytes to seal the wound bed from the environment. However, the signaling events that coordinate this collective migration are unclear. In this report, we address the role of phosphorylation of
Noa Aharon-Hefetz et al.
eLife, 9 (2020-12-29)
Different subsets of the tRNA pool in human cells are expressed in different cellular conditions. The 'proliferation-tRNAs' are induced upon normal and cancerous cell division, while the 'differentiation-tRNAs' are active in non-dividing, differentiated cells. Here we examine the essentiality of
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