Skip to Content
Merck
All Photos(1)

Documents

M6395

Sigma-Aldrich

MCDB 105 Medium

With trace elements, L-glutamine and 25mM HEPES, powder, suitable for cell culture

Synonym(s):

Molecular, Cellular, and Development Biology 105 medium

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

phenol red: 0.001242 g/L
glucose: 0.72064 g/L (Dextro)
sodium pyruvate: 0.11 g/L
HEPES: 5.958 g/L (25mM)
L-glutamine: 0.3653 g/L

shipped in

ambient

storage temp.

2-8°C

Related Categories

General description

MCDB media has been designed for the low-protein or serum-free growth of specific cell types using hormones, growth factors, trace elements, or low levels of dialyzed fetal bovine serum protein (FBSP). Each MCDB medium is formulated (qualitatively and quantitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes the growth of a specific cell type. MCDB 105 is derived from the modifications done with MCDB 104 medium. This medium is suitable for the long-term growth of WI-38, MRC-5, and other human diploid fibroblast-like cells.

Application

MCDB 105 Medium has been used to culture:
  • human umbilical vein endothelial cells
  • primary human endometrial stromal cells (ESC)
  • ovarian cancer cell line (OVCAR3)

Quantity

Formulated to contain 14.9 grams of powder per liter of medium.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Bjørn Grinde et al.
Virus research, 123(1), 86-94 (2006-09-26)
Polyomavirus BK-associated nephropathy (PVAN) is an emerging cause of early renal transplant failure. In order to learn more about the cellular response to BK virus, microarrays were used to study its effect on mRNA expression in human endothelial cells. The
Minghai Shao et al.
Nucleic acids research, 39(10), 4035-4047 (2011-01-27)
Platelet derived growth factor (PDGF) regulates gene transcription by binding to specific receptors. PDGF plays a critical role in oncogenesis in brain and other tumors, regulates angiogenesis, and remodels the stroma in physiologic conditions. Here, we show by using microRNA
Jana Kim et al.
Journal of magnetic resonance imaging : JMRI, 47(6), 1589-1600 (2017-12-06)
Steady state susceptibility contrast (SSC)-MRI provides information on vascular morphology but is a rarely used method. To investigate the utility of the ultrasmall superparamagnetic iron oxide particles (USPIOs) GEH121333 for measuring tumor response to bevacizumab and compare this with gadolinium-based
Jing-Jing Wang et al.
Oncoimmunology, 8(11), e1659092-e1659092 (2019-10-28)
Blockade of the programmed cell death 1(PD-1)/PD-1 ligand-1(PD-L1) pathway has been exploited therapeutically in many cancer types. Upregulation of PD-L1 in tumor cells contributes to malignancy through suppression of the T cell-mediated antitumor response. Pyruvate dehydrogenase kinase 1 (PDK1), a
Marta Llauradó Fernández et al.
American journal of cancer research, 6(10), 2235-2251 (2016-11-09)
Advanced or recurrent low-grade serous ovarian cancers (LGSC) are resistant to conventional systemic treatments. LGSC carry mutations in RAS or RAF, leading to several clinical trials evaluating MEK inhibitors (MEKi). As LGSC cell lines and xenografts have been difficult to
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service