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L8275

Sigma-Aldrich

Lectin from Galanthus nivalis (snowdrop)

lyophilized powder

Synonym(s):

Galanthus nivalis lectin, GNL

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About This Item

MDL number:
MFCD00163718
UNSPSC Code:
12352202
NACRES:
NA.32
Pricing and availability is not currently available.

form

lyophilized powder

Quality Level

200

potency

<20 μg per mL agglutination activity

composition

Protein, ≥90% Lowry

impurities

salt, essentially free

storage temp.

2-8°C

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General description

Lectin from Galanthus nivalis (snowdrop) is a tetrameric, 12 kDa mannose binding lectin.[1] It belongs to the family, Amaryllidacea.[2] It is specific to α-1,3- or 1,6- linked D-mannose of carbohydrates. Snowdrop lectins are toxic to insects.[1]

Application

Lectin from Galanthus nivalis (snowdrop) has been used:
  • to coat ELISA plates[3]
  • as reverse transcriptase inhibitor[4]
  • in ELISA for detection of envelope glycoproteins of the human and simian immunodeficiency viruses[5]

Biochem/physiol Actions

GNL agglutinates rabbit erythrocytes; human erythrocytes are not agglutinated. GNL sugar specificity is directed to the nonreducing end of the terminal α-D-mannosyl residue of glycoconjugates.
Lectin from Galanthus nivalis (snowdrop) provide resistance against pests and are used in transgenic plants.[6] Snowdrop lectin functions as a carrier to transport fused proteins to the hemolymph as it crosses gut epithelium of target species to exhibit insecticidal effects.[7]

Preparation Note

Purified by affinity chromatography according to Kaku.[2]

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilution in phosphate buffered saline, pH 7.2 of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of rabbit erythrocytes after 1 hr incubation at 25 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000262504
0000271667
0000271666
0000271668
0000236787

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Snowdrop lectin.
H Kaku et al.
Methods in enzymology, 179, 327-331 (1989-01-01)
A model for the hepatitis C virus envelope glycoprotein E2
Yagnik AT, et al.
Proteins: Structure, Function, and Bioinformatics, 40, 355-366 (2000)
Direct effects of snowdrop lectin (GNA) on larvae of three aphid predators and fate of GNA after ingestion
Hogervorst PAM, et al.
Journal of Insect Physiology, 52, 614-624 (2006)
Jacklyn Johnson et al.
Journal of virology, 94(9) (2020-02-28)
Semen is the primary transmission vehicle for various pathogenic viruses. Initial steps of transmission, including cell attachment and entry, likely occur in the presence of semen. However, the unstable nature of human seminal plasma and its toxic effects on cells
Comparison of serological tests for the diagnosis of feline immunodeficiency virus infection of cats
Sibille P, et al.
Veterinary Microbiology, 45(2-3), 25-267 (1995)
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