GE17-5445-02
Percoll® PLUS
Cytiva 17-5445-02, pack of 250 mL
About This Item
Recommended Products
sterility
non-sterile (LOW ENDOTOXIN (2 EU/ml))
form
colloidal (Colloidal solution of silica particles coated with silane)
liquid (Suspension)
solution
packaging
pack of 250 mL
manufacturer/tradename
Cytiva 17-5445-02
color
Colorless to slightly yellow
pH range
8.9-9.9
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Related Categories
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This Item | CHP-520 | CHP-502 | CHP-503 |
---|---|---|---|
10 mM | 10 mM | 10 mM | 10 mM |
100 | 100 | 100 | 100 |
100 mol % 12:0 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC) | 30 mol % Cholesterol, 70 mol % 18:0 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) | 100 mol % 15:0 1,2-dipentadecanoyl-sn-glycero-3-phosphocholine | 100 mol % 16:0 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) |
100 nm | 100 nm | 100 nm | 100 nm |
7.4 | 7.4 | 7.4 | 7.4 |
General description
Cell separation with the medium is performed under gentle conditions, facilitating the isolation of a variety of cells, subcellular particles, and viruses where preservation of viability and morphological integrity is important. The low toxicity of Percoll PLUS ensures that removal of the medium from separated cellular particles is not usually necessary.
After adjustment, Percoll PLUS forms iso-osmotic gradients within the density range of 1.0 to 1.3 g/ml. This density range is especially useful since most cells, subcellular particles, and viruses have a buoyant density of 1.0 to 1.2 g/ml in Percoll PLUS.
Application
The medium is also suited to applications where high-speed centrifugation is required. In this case, the sample can be premixed with the medium and subsequently separated on the continuous gradient formed in situ. Thus, gradient
formation and sample separation can be achieved in one step.
All experiments described in the literature using Percoll® can also be performed with Percoll PLUS.
Features and Benefits
- Low endotoxin levels (max. 2 EU/ml)
- Absence of toxicity for cells and very low chemical reactivity
- Low osmolality: Percoll PLUS can easily be adjusted with physiological saline, other balanced salt solutions, or cell culture media, to give gradients that are iso-osmotic throughout
- Low viscosity resulting in rapid formation of gradients and particle separation at low centrifugal forces
Storage and Stability
Analysis Note
Legal Information
Storage Class Code
12 - Non Combustible Liquids
Certificates of Analysis (COA)
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