E1655

Anti-phospho-Epidermal Growth Factor Receptor [pTyr¹¹⁴⁸] antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

• Synonym(s): Anti-phospho-EGFR [pTyr¹¹⁴⁸]
• MDL number: MFCD03845327
• UNSPSC Code: 51111800
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous glycerol solution
• mol wt: antigen ~170 kDa
• species reactivity: human
• technique(s): western blot: 1:1,000 using human epithelial A431 cells stimulated with EGF or mouse hybridoma NIH3T3 cells expressing human EGFR and stimulated with EGF
• UniProt accession no.: P00533
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... EGFR(1956)

Description

General description

The members of epidermal growth factor receptor (EGF R) or the ErbB receptor family have been identified as useful biomarkers and targets for cancer therapy. The EGFR family includes four receptor tyrosine kinases, EGF R (ErbB1), ErbB2 (neu), ErbB3, and ErbB4. EGF R binds EGF and induces tyrosine phosphorylation leading to proliferation of cells. EGF R is present on many cell types of epithelial and mesenchymal lineages. EGF R is capable of binding transforming growth factor-α and heparin-binding EGF in addition to EGF. There are numerous effector molecule activated by EGF R that result in a variety of biological processes such as morphogenesis, cell motility, apoptosis, differentiation and organ repair and maintenance. Deregulation of EGF R signaling is implicated in progression of a wide variety of tumors, invasion and metastasis. Phosphorylation of EGF R at Tyr1148 is by autophosphorylation may be required for the binding of protein tyrosine phosphatase, SHP-1, which results in dephosphorylation of EFG R
Anti-phospho-EGFR [pTyr¹¹⁴⁸] specifically recognizes human EGFR phosphorylated at tyrosine 1148.

Immunogen

synthetic phosphopeptide derived from the region of EGFR that contains tyrosine 1148.

Application

Anti-phospho-EGFR [pTyr¹¹⁴⁸] antibody may be used at a working dilution of 1:1000 for immunoblotting using A431 cells stimulated with EGF.

Physical form

Solution in Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3, with 50% glycerol, 1.0 mg/mL BSA (IgG, protease free) as a carrier and 0.05% sodium azide.

Safety Information

• Storage Class Code: 10 - Combustible liquids