CHA00011B
EUR-2019-nCoV RdRp D for detection of SARS-CoV-2 RNA
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About This Item
UNSPSC Code:
41105500
NACRES:
NA.51
form
solution (in 10 mM Tris, 1 mM EDTA)
packaging
pkg of 1 vial
pH range
7.5-8.5
shipped in
ambient
storage temp.
−20°C
General description
EUR-2019-nCoV Modified RdRp (formerly Discrimatory) Individual Assay is for qPCR (5′ nuclease assay) detection of SARS-CoV-2.
The product is primers and probes, the sequences of which were designed and made publicly available by Charité Institute of Virology – Universitätsmedizin Berlin.
For additional details, please visit SigmaAldrich.com/ncov.
The product is primers and probes, the sequences of which were designed and made publicly available by Charité Institute of Virology – Universitätsmedizin Berlin.
For additional details, please visit SigmaAldrich.com/ncov.
Features and Benefits
The forward and reverse primers in each tube come with the following features:
The Dual-Labeled Probe in each tube comes with the following features:
- DNA bases
- No modifications
- Desalt purified
- 10 nmol each
The Dual-Labeled Probe in each tube comes with the following features:
- DNA bases
- 6-FAM™ reporter
- BXQ (Block All Quencher—SigmaAldrich.com/bxq)
- HPLC purified
- 5nmol
Components
The product includes 1 individual tube of a 20X primer and probe pool for quantification of a SARS-CoV-2 gene. Assay included is Modified RdRp.
The forward primer, reverse primer, and probe are at final concentrations of 10, 10, and 5 μM, respectively.
Modified RdRp Assay:
Forward Primer AAATGGTCATGTGTGGCGGT
Reverse Primer GTTAAAAACACTATTAGCATAAGCAGTTGT
Dual-Labeled Probe CAGGTGGAACCTCATCAGGAGATGC
The forward primer, reverse primer, and probe are at final concentrations of 10, 10, and 5 μM, respectively.
Modified RdRp Assay:
Forward Primer AAATGGTCATGTGTGGCGGT
Reverse Primer GTTAAAAACACTATTAGCATAAGCAGTTGT
Dual-Labeled Probe CAGGTGGAACCTCATCAGGAGATGC
Legal Information
FAM is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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