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46985

Sigma-Aldrich

Fluorescein-5-thiosemicarbazide

suitable for fluorescence, ~80% (HPCE)

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About This Item

Empirical Formula (Hill Notation):
C21H15N3O5S
CAS Number:
Molecular Weight:
421.43
MDL number:
UNSPSC Code:
12352125
PubChem Substance ID:
NACRES:
NA.32

Assay

~80% (HPCE)

Quality Level

form

solid

solubility

DMF: soluble
H2O: soluble

fluorescence

λex 492 nm; λem 516 nm in 0.1 M Tris pH 9.0

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

NNC(=S)Nc1ccc(c(c1)C(O)=O)C2=C3C=CC(=O)C=C3Oc4cc(O)ccc24

InChI

1S/C21H15N3O5S/c22-24-21(30)23-10-1-4-13(16(7-10)20(27)28)19-14-5-2-11(25)8-17(14)29-18-9-12(26)3-6-15(18)19/h1-9,25H,22H2,(H,27,28)(H2,23,24,30)

InChI key

MEUCHQDLZYLNQY-UHFFFAOYSA-N

General description

Fluorescein-5-thiosemicarbazide, also known as FTC, is a fluorescent probe. The FTSC fluorescent spectra is like FITC, a preferred fluorescent tag for proteomics. The fluorescence spectral properties of FTSC-labeled vesicles obtained by reductive amination are fully compatible with the argon laser of fluorescent instruments.

Application

Fluorescein-5-thiosemicarbazide can be used as a fluorescent labeling reagent to label:
  • Cell-surface functional groups (glycophorins) in the study of the effect of deoxygenation in the red blood cell membrane.
  • Saccharides applicable in polysaccharide imaging in live cells.
  • Chondroitin sulfate nanogels applicable in cell-specific drug delivery applications.

Features and Benefits

Fluorescein-5-thiosemicarbazide has the following benefits -

  • It displays strong fluorescence property.
  • Shows an intrinsic reactivity of the thiosemicarbazide group towards the aldehyde group.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Kylan Szeto et al.
PloS one, 8(12), e82667-e82667 (2014-01-01)
Aptamers are high-affinity ligands selected from DNA or RNA libraries via SELEX, a repetitive in vitro process of sequential selection and amplification steps. RNA SELEX is more complicated than DNA SELEX because of the additional transcription and reverse transcription steps.
Seiichi Ohta et al.
The Journal of surgical research, 242, 286-295 (2019-05-28)
Adhesion formation is a critical issue in surgery, particularly in hepatectomy. The present study aimed to develop a bilayer adhesion barrier comprising alginate (Alg) of different molecular weight (Mw). It was expected that a slowly dissolving layer remains on the
Ying Zhang et al.
Carbohydrate research, 346(14), 2156-2164 (2011-08-10)
A simple and efficient procedure for the fluorescent labeling of saccharides is a prerequisite step for imaging the transport of polysaccharides in living cells. We report a one-pot strategy for the fluorescent labeling of saccharides with fluorescein-5-thiosemicarbazide (FTSC), which introduces
M Havé et al.
Plant biology (Stuttgart, Germany), 17(5), 973-979 (2015-02-17)
Leaf senescence is characterised by a massive degradation of proteins in order to recycle nitrogen to other parts of the plant, such as younger leaves or developing grain/seed. Protein degradation during leaf senescence is a highly regulated process and it
Injectable hydrogel-incorporated cancer cell-specific cisplatin releasing nanogels for targeted drug delivery
Gil MS, et al.
Journal of Material Chemistry B: Materials for Biology and Medicine, 5(34), 7140-7152 (2017)

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