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Key Documents

ABC118

Sigma-Aldrich

Anti-phospho Beclin Antibody (Thr119)

from rabbit, purified by affinity chromatography

Synonym(s):

Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

rat (based on 100% sequence homology), Xenopus (based on 100% sequence homology), bovine (based on 100% sequence homology), chicken (based on 100% sequence homology), mouse (based on 100% sequence homology), porcine (based on 100% sequence homology)

technique(s)

inhibition assay: suitable (peptide)
western blot: suitable

NCBI accession no.

NP_003757

UniProt accession no.

Q14457

shipped in

wet ice

target post-translational modification

phosphorylation (pThr119)

Gene Information

human ... BECN1(8678)

General description

The Beclin-1 (BECN1) gene encodes a 60 kDa coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2. Beclin-1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin-1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity, tumorigenesis, and has been found to ameliorate the neurodegenerative pathology in α-Synuclein models of Parkinson′s and lewy body diseases. Endogenous Beclin-1 protein expression is frequently low in human breast epithelial carcinoma cell lines and tissue, but is expressed ubiquitously at high levels in normal breast epithelia. It has also been shown that Beclin 1 contains a leucine-rich nuclear export signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport.

Specificity

This antibody recognizes the BH3 domain of Beclin phosphorylated at Thr119.

Immunogen

Epitope: BH3 domain
KLH-conjugated linear peptide corresponding to the BH3 domain of human Beclin phosphorylated at Thr119.

Application

Detect Beclin using this rabbit polyclonal antibody, Anti-phospho Beclin Antibody (Thr119) validated for use in western blotting & Peptide Inhibition Assay.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Western Blotting/Peptide Inhibition Analysis: 5 µg/mL from a representative lot detected phospho Beclin (Thr119) in LNCap cell lysate. Peptide inhibition was observed with the addition of phospho peptides, but not with the addition of non-phospho peptides.

Quality

Evaluated by Western Blotting in human prostate tumor tissue lysate.

Western Blotting Analysis: 5 µg/mL of this antibody detected 10 µg of human prostate tumor tissue lysate.

Target description

~50 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Physical form

Affinity purified
Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human prostate tumor tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Miray Turk et al.
Molecules (Basel, Switzerland), 26(4) (2021-02-11)
Expression levels of the major mammalian autophagy regulator Beclin 1 and its interaction with Bcl-2 regulate the switch between autophagic cell survival and apoptotic cell death pathways. However, some of the regulators and the precise mechanisms of these processes still
M-L Plissonnier et al.
Oncogene, 36(48), 6712-6724 (2017-08-08)
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Changxuan Chen et al.
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, 51(6), e7080-e7080 (2018-04-26)
Prostate cancer (PCa) is the second leading cause of cancer death in men. Irradiation is one of the available options for treatment of PCa, however, approximately 10-45% of PCa are resistant to irradiation. We aimed to explore the role of
Hanan Abu-Tayeh Suleiman et al.
International journal of molecular sciences, 23(20) (2022-10-28)
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Yajing Wang et al.
Autophagy, 13(11), 1855-1869 (2017-08-22)
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