569224-U
Discovery® C18 (5 µm) HPLC Columns
L × I.D. 25 cm × 10 mm, HPLC Column
Synonym(s):
Discovery RP18 HPLC Column
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
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product name
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 10 mm
material
stainless steel column
Agency
suitable for USP L1
product line
Discovery®
feature
endcapped
manufacturer/tradename
Discovery®
packaging
1 ea of
extent of labeling
12% Carbon loading
parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
L × I.D.
25 cm × 10 mm
surface area
300 m2/g
surface coverage
3 μmol/m2
impurities
<10 ppm metals
matrix
silica gel, high purity, spherical particle platform
fully porous particle
matrix active group
C18 (octadecyl) phase
particle size
5 μm
pore size
180 Å
operating pH range
2-8
application(s)
food and beverages
separation technique
reversed phase
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General description
Use Discovery® C18 for any method that specifies a C18. The exceptional peak shape, reproducibility, and stability make it the column of choice for all C18 methods from demanding to routine.
Application
- Development and Validation of a Reversed-Phase HPLC Method with UV Detection for the Determination of L-Dopa in Vicia faba L. Broad Beans.: Highlights the application of the Discovery® C18 HPLC Column in developing a method for the accurate quantification of L-Dopa in natural sources, which is essential for studies in neuropharmacology and natural product research (Tesoro et al., 2022).
- Simultaneous estimation of paclitaxel and erlotinib in plasma by liquid chromatography/(+) electrospray tandem mass spectrometry: application in formulation development and pharmacokinetics.: This study employs the Discovery® C18 HPLC Column to enhance the simultaneous analysis of paclitaxel and erlotinib, critical for cancer therapy research and formulation development (Khuroo et al., 2022).
Features and Benefits
- Excellent reproducibility
- Exceptional peak shape for basic and acidic analytes
- Stable, low-bleed LC-MS separations
- Separation of peptides and small proteins
- Lower hydrophobicity than many comparable C18 columns, providing faster analysis
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
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Yoshinori Masukawa et al.
Journal of lipid research, 49(7), 1466-1476 (2008-03-25)
Ceramides (CERs) in human stratum corneum (SC) play physicochemical roles in determining barrier and water-holding functions of the skin, and specific species might be closely related to the regulation of keratinization, together with other CER-related lipids. Structures of those diverse
Pei Yuan et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 15(42), 11319-11325 (2009-09-18)
This study systematically investigates periodic mesoporous organosilicas (PMOs) with controlled helical and concentric circular (CC) pore architectures prepared through a basic-catalyzed sol-gel process by using an achiral cationic surfactant trimethyloctadecylammonium bromide (C(18)TAB) as a structure-directing agent, perfluorooctanoic acid (PFOA) as
R Nageswara Rao et al.
Biomedical chromatography : BMC, 23(11), 1145-1150 (2009-05-16)
A highly sensitive and selective on-line two-dimensional reversed-phase liquid chromatography/electrospray ionization-tandem mass spectrometry (2D-LC-ESI/MS/MS) method was developed and validated to determine rifaximin in rat serum by direct injection. The 2D-LC-ESI/MS/MS system consisted of a restricted access media column for trapping
Pilar Heredia-Middleton et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 149(4), 552-556 (2008-02-01)
Heat shock protein 70 (HSP70, 70 kDa) is the most commonly expressed protein in response to thermal stress. The extent of its expression is associated with differences in environmental temperatures. We investigated the heat shock response in red blood cells
S Bartesaghi et al.
Journal of pharmaceutical and biomedical analysis, 24(5-6), 1137-1141 (2001-03-15)
Total plasma homocysteine (tHcy) in children may be an useful biochemical marker for genetic risk of premature cardiovascular disease. We reported a rapid, isocratic HPLC method able to process very small amount of newborn plasma samples. A blood sample from
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