SRP2116

RNA Polymerase II, p15.6 subunit, GST tagged human

recombinant, expressed in E. coli, ≥85% (SDS-PAGE)

• Synonym(s): HRBP14.4, POLRF, RPABC2, RPB14.4, RPB6
• UNSPSC Code: 12352202
• NACRES: NA.26

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥85% (SDS-PAGE)
• form: frozen liquid
• mol wt: ~40 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: 200 μg/mL
• color: clear colorless
• NCBI accession no.: NM_021974
• UniProt accession no.: P61218
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... POLR2F(5435)

Description

Biochem/physiol Actions

hRPB6 is a highly conserved subunit shared by all three RNA polymerases, consisting of 142 amino acid residues. The gene for yeast RPB6 is essential for cell viability and homologues of this subunit exist in archaeal and some viral RNA polymerases. For example the bacterial w (the fifth subunit of the bacterial RNAP core enzyme) and eukaryotic RPB6 are structural homologs. RPB6 has been shown to promote pol II complex assembly, and/or increase its stability, through specific interactions with the largest subunit of RNA pol II. In addition, RPB6 was found to make contact with three small subunits, RPB5, RPB7, and RPB8 and to play a role in the interaction between RNA polymerase II and the transcription elongation factor TFIIS.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable