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M7431

Sigma-Aldrich

Monoclonal Anti-MAP Kinase 2 (ERK-2) antibody produced in mouse

clone 1B3B9, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-ERK, Anti-ERK-2, Anti-ERT1, Anti-MAPK2, Anti-NS13, Anti-P42MAPK, Anti-PRKM1, Anti-PRKM2, Anti-p40, Anti-p41, Anti-p41mapk, Anti-p42-MAPK

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.44

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1B3B9, monoclonal

form

buffered aqueous solution

mol wt

antigen 42 kDa

species reactivity

human, rat, mouse

technique(s)

immunoprecipitation (IP): 4 μg using denatured ERK-2 from a mouse 3T3 fibroblast lysate
microarray: suitable
western blot: 0.5 μg/mL using lysates from human A431 cells, mouse 3T3 fibroblasts or rat L6 cells

isotype

IgG2a

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MAPK1(5594)
mouse ... Mapk1(26413)
rat ... Mapk1(116590)

General description

Mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in widespread signalling pathways. Members of this family include the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), JNK and p38 MAPK subfamilies. These are the terminal enzymes in a signalling cascade where each kinase phosphorylates and activates the next member in the sequence. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. Several kinases participate in activation of the ERK cascade. This cascade is initiated by the small G protein Ras, which upon stimulation causes activation Raf1 kinase. Raf1 continues the transmission by activating MEK. Activated MEK appears to be the only kinase capable of specifically phosphorylating and activating ERK. ERK appears to be an important regulatory molecule, which by can phosphorylate regulatory targets in the cytosol (phospholipase A2, PLA2), translocated into and phosphorylate substrates in the nucleus (ELK1). The activation of ERK cascade mediates and regulates the signal transduction pathways in response to stress, mitogenic signals and is important in development and differentiation, learning, memory and survival.
Anti-MAP Kinase 2 recognizes the 42 kDa MAPK2 encoded by the mapk gene. The antibody may weakly react with MAPK1 (ERK1) and only binds denatured MAP kinase.

Immunogen

recombinant mouse MAP kinase.

Application

Monoclonal Anti-MAP Kinase 2 (ERK-2) antibody is suitable for detection by immunoblotting, at a working concentration of 0.5 μg/mL, using whole cell lysates of human A431, mouse 3T3 fibroblasts or rat L6 cells. Detection of MAPK2 (ERK-2) was possible in human colon adenocarcinoma cells, HT-29. Immunoprecipitation applications in mouse 3T3 fibroblasts require a concentration of 4 μg. The antibody may be suitable for protein microarray.

Physical form

Solution in phosphate buffered saline containing 0.05% sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Huayun Deng et al.
FEBS letters, 585(12), 1957-1962 (2011-05-24)
GPR35 is an orphan G protein-coupled receptor that is not well-characterized. Here we employ dynamic mass redistribution (DMR) assays to discover new GPR35 agonists. DMR assays identified tyrphostin analogs as GPR35 agonists, which were confirmed with receptor internalization, Tango β-arrestin
Targeting the Extracellular Signal-Regulated Kinase Pathway in Cancer Therapy
Tanimura S and Ozaki K
Molecular Biology of the Cell, 34, 1781-1784 (2011)
Haibei Hu et al.
PloS one, 7(4), e34934-e34934 (2012-04-19)
Fluorescent and luminescent probes are essential to both in vitro molecular assays and in vivo imaging techniques, and have been extensively used to measure biological function. However, little is known about the biological activity, thus potential interferences with the assay
The mitogen-activated protein kinase signal transduction pathway.
R J Davis
The Journal of biological chemistry, 268(20), 14553-14556 (1993-07-15)
R J Davis
Trends in biochemical sciences, 19(11), 470-473 (1994-11-01)
Mitogen-activated protein kinases (MAPKs) are activated by dual phosphorylation on threonine and tyrosine in response to a wide array of extracellular stimuli. In the yeast Saccharomyces cerevisiae, a series of extracellular stimuli. In the yeast Saccharomyces cerevisiae, a series of

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