Whole genome CRISPR knockout screening for every lab

• Utilize the power of CRISPR to knockout and screen every gene in the human genome
• Select from the GeCKOv2 or our Whole Human Genome CRISPR Pools
• Average of 6 gRNAs per gene (GeCKOv2); 10 gRNAs per gene
• Divided into 2 subpools at 62,000 clones per pool (GeCKOv2, 124,000 clones total);8 subpools at 23,000 clones per pool (184,000 clones total)
• Numerous built-in enrichment, depletion, and non-targeting control clones allow researchers to confidently gauge the success of their pooled screening experiments
• 2 vector system (pools are gRNA-only, Cas9 sold separately)
• All pools are deep sequenced to verify clone representation and distribution
• Deliverables: 200 μl of each pool at a minimum of 5x108 viral particles/ml (via p24), ships immediately

Create Your Own CRISPR Pool - Focus on the genes essential to your research

• Special promotion: 10,000 pre-designed clones for $12,000
• Custom viral titers, volumes, and aliquoting are available (standard deliverable: 200 μl at a minimum of 5 x 10⁸ viral particles/ml in 4 x 50 μl aliquots); quotes available upon request
• All pools are deep sequenced to verify clone representation and distribution
• TAT: 8-10 weeks from finalization of clone list
• DNA format also available

Survey the entire human kinome for novel hits with pooled CRISPR knockout clones

• Over 6,000 unique lentiviral clones expressing both Cas9 and gRNA sequences designed to functionally inactivate (knockout) nearly 700 human kinase genes
• High clone coverage (7-10 distinct gRNAs per kinase gene, average of 8.6 per gene) increases the statistical strength of your hit analysis
• Numerous built-in enrichment and depletion controls
• The pool contains 100 non-targeting control gRNAs as normalization standards
• The human kinase pool is deep sequenced to verify clone representation and distribution
• gRNA design and tiling was created with a stringent bioinformatics approach to minimize the potential for off-targeting

Knockdown the genome

• Trusted TRC (The RNAi Consortium) content with shRNAs targeting 20,000+ human or 21,000+ mouse genes including TRC 1.5 and 2.0 (unique and validated) add-ons which are exclusive to us
• Enjoy the power of lentiviral transduction for screening in standard or difficult to expand cell lines.
• Deliverables: 50 μl of each pool at a minimum of 5x10⁸ viral particles/ml (via p24), ships immediately
• Versatile and compatible with FACS and most downstream fluorescent assays
• Affordable and easy-to-use allowing for bench-top discovery without robotics or liquid handling

Focus on the genes essential to your research by creating your own custom shRNA pool

• Create a custom shRNA pool to specifically target your genes of interest
• Customize your viral titers, volumes, and aliquoting needs for greater flexibility in your screening experiments. DNA format also available.
• Titers and volumes adequate for in vitro, in vivo and xenograft applications
• Partner with our next-generation sequencing services for data deconvolution to get the most out of your assays

Knockdown and focus on the kinase gene pathway

• Over 9,400 unique lentiviral shRNA clones to knockdown 740+ human kinase genes
• High clone coverage (average of 12.6 clones per gene) increases the statistical strength of your hit analysis
• Deliverables: 100 μl of each pool at a minimum of 5x10⁸ viral particles/ml (via p24), ships immediately
• Numerous built-in enrichment, depletion, and non-target control clones allows researchers to confidently gauge the success of their pooled screening experiments

Whole genome overexpression ORF screening at a fraction of the cost

• Exclusive to us - Puromycin expressing LentiORF library barcoded for pooled screening applications
• Each ORF Clone contains a unique 24 NT barcode sequence for easy deconvolution and identification of hits
• Perform overexpression screening to study cancer phenotypes
• Trusted TRC3 (The RNAi Consortium) content with ORF gene expression spanning 14,000+ human genes and 17,000+ clones
• Simplified whole genome ORF screening in standard or difficult to expand cell lines