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C8195

Sigma-Aldrich

Complement C4 from human serum

≥300,000 C4H50 units/mg protein

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About This Item

CAS Number:
80295-48-3
MDL number:
MFCD00130838
UNSPSC Code:
12352202
NACRES:
NA.61
Pricing and availability is not currently available.

biological source

human serum

Quality Level

200

form

solution

specific activity

≥300,000 C4H50 units/mg protein

technique(s)

activity assay: suitable

UniProt accession no.

P0C0L4

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... C4A(720)

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General description

Complement C4 is mapped to human chromosome 6p21.33. It exists in two isoforms as c4A and c4B.[1] C4 is a 200 kDa protein belonging to α2 macroglobulin (α2M) family. It undergoes posttranslational modification like sulfation and glycosylation and finally proteolysis to become a mature protein.[2]

Application

Complement C4 from human serum has been used in mannose binding lectin (MBL) complex assay of serum samples[3] and in MBL-associated serine proteases based C4 activation assay of plasma samples.[4]

Biochem/physiol Actions

Complement C4 serves a primary role in the classical and lectin pathways of the complement system and provides protection against bacterial pathogens. C4 polymorphisms have been used in research to study their associations with the hemolytic and antibacterial ability of the classical pathway.[5][6]
There are strong indications that complement C4 deficiency is correlated with induction of systemic lupus erythematosus (SLE) and other autoimmune diseases.
Variation in the copy number of the complement C4 gene alters the complement c4A and c4B levels in age-related macular degeneration (AMD)[7] and increases risk of developing schizophrenia.[8]

Physical form

Supplied as a solution in PBS, pH 7.2

Other Notes

View more information on the complement pathway at www.sigma-aldrich.com/enzymeexplorer

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCM7811
SLCH8255
SLCF0437
SLBW9849
SLBN3829V

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Yan Yang et al.
Current directions in autoimmunity, 7, 98-132 (2004-01-15)
It was observed about 50 years ago that low serum complement activity or low protein concentrations of complement C4 concurred with disease activities of systemic lupus erythematosus (SLE). Complete deficiencies of complement components C4A and C4B, albeit rare in human
Genetic, structural and functional diversities of human complement components C4A and C4B and their mouse homologues, Slp and C4
Blanchong CA, et al.
International Immunopharmacology, 1(3), 365-392 (2001)
Structural basis for the function of complement component C4 within the classical and lectin pathways of complement
Mortensen S, et al
Journal of Immunology (2015)
Structural basis for activation of the complement system by component C4 cleavage
Kidmose RT, et al.
Proceedings of the National Academy of Sciences of the USA, 109, 15425-15430 (2012)
Multiallelic copy number variation in the complement component 4A (C4A) gene is associated with late-stage age-related macular degeneration (AMD)
Grassmann F, et al.
Journal of Neuroinflammation, 13(1), 81-81 (2016)
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Questions

1–3 of 3 Questions  

  1. How does the storage temperature relate to shipping conditions?

    1 answer

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

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  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  3. What is the molecular weight of Product C8195, Complement C4 from human serum?

    1 answer

    1. The molecular weight for Product C8195, Complement C4 from human serum, is 205,000 daltons as described in Complement: A Practical Approach, A.W. Dodds and R.B. Sim, Eds., p. 2, 1997.

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