S2255

Safranin O

Dye content ≥85 %, Powder

• Synonym(s): Basic Red 2, Cotton Red, Gossypimine, Safranin T, Safranin Y or A
• Empirical Formula (Hill Notation): C₂₀H₁₉ClN₄
• CAS Number: 477-73-6
• Molecular Weight: 350.84
• Colour Index Number: 50240
• Beilstein: 3924099
• EC Number: 207-518-8
• MDL number: MFCD00011759
• UNSPSC Code: 12171500
• PubChem Substance ID: 24899516
• NACRES: NA.47

Properties

• Product Name: Safranin O, Dye content ≥85 %
• form: powder
• Quality Level: 200
• composition: Dye content, ≥85%
• technique(s): microbe id | staining: suitable
• pH: 10 (20 °C, 10 g/L)
• solubility: water: 1 mg/mL, clear, dark red to very dark red and red purple
• εmax: 1250-1650 at 530-534 nm in 50% ethanol
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N
• InChI: 1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H
• InChI key: OARRHUQTFTUEOS-UHFFFAOYSA-N

Description

General description

Safranin O is a cationic dye, which displays less significant metachromasia. It is used to detect any variations in articular disease. Safranin O binds specifically to polyanions. It detects the amount of proteoglycan present in cartilages. Safranin O is used as a marker for glycosaminoglycan depletion in osteoarthritis. Safranin is used as a counter stain in Gram staining.

Application

Safranin O has been used in the histological analysis of femurs. It has also been used to detect the presence of glycosaminoglycans in the inner body of meniscus structures.

Safety Information

• Pictograms: GHS05
• Signal Word: Danger
• Hazard Statements: H318
• Precautionary Statements: P280 - P305 + P351 + P338
• Hazard Classifications: Eye Dam. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves