P3504

Ponceau S

Powder

• Synonym(s): 3-Hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt
• Empirical Formula (Hill Notation): C₂₂H₁₂N₄Na₄O₁₃S₄
• CAS Number: 6226-79-5
• Molecular Weight: 760.57
• Colour Index Number: 27195
• EC Number: 228-319-2
• MDL number: MFCD00003892
• UNSPSC Code: 12171500
• PubChem Substance ID: 24898418
• NACRES: NA.47

Properties

• product name: Ponceau S, BioReagent, suitable for electrophoresis
• product line: BioReagent
• Quality Level: 200
• form: powder
• technique(s): electrophoresis: suitable
• color: red to very dark red
• solubility: H₂O: 1 mg/mL
• ε (extinction coefficient): ≥330000 at 518-522 nm in deionized water at 0.015 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O
• InChI: 1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;
• InChI key: KQHKSGRIBYJYFX-WNDMICSESA-J

Description

General description

Ponceau S is the sodium salt of a diazo dye. It is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. The stain is reversed with water washes and aids in subsequent immunological detection.
The following are two common stain formulations:
0.1% Ponceau S (w/v) in 5% acetic acid
2% Ponceau S (w/v) in 30% TCA, 30% sulfosalicylic acid

Application

Ponceau S has been used for membrane staining (Western blotting.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves