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96351

Supelco

Pullulan Standard Set Mp ~350 - 700′000

set of analytical standards, for GPC

Synonym(s):

Pullulan Standard Set

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About This Item

CAS Number:
9057-02-7
EC Number:
232-945-1
MDL number:
MFCD00027321
UNSPSC Code:
41116107
E Number:
E1204
NACRES:
NA.24
Pricing and availability is not currently available.

grade

analytical standard
for GPC

shelf life

limited shelf life, expiry date on the label

analyte chemical class(es)

oligosaccharides

technique(s)

gel permeation chromatography (GPC): suitable

application(s)

cleaning products
cosmetics
food and beverages
personal care

format

neat

storage temp.

2-8°C

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General description

Pullulan is a linear glucan polysaccharide made up of α-1,4-maltotriose repeating units. It is produced by yeast-like fungus Aureobasidium pullulans from starch and has α-1,6 linkages between two maltotriose units.[1]

Application

The set of analytical standards can also be used as follows:
  • Calibration of the polymer-based column in the estimation of molecular weight of the polysaccharide fractions obtained from seaweeds— S. latissima and M. stellatus, by a high-performance size exclusion chromatography (HPSEC) method
  • Measurement of molecular masses of oligomeric cellulose by size exclusion chromatography in combination with multi-angle laser light scattering (MALLS) detection
  • To calibrate the SEC column in the determination of molecular size distribution of microalgal native glycogen
  • Quantitative analysis of membrane formation process of alginate–chitosan microcapsules using gel permeation chromatography(GPC).[2]

Components

Kit with carefully selected reference materials to calibrate GPC/SEC/GFC systems. The kit contains standards of the same type, but with different molar masses, a certificate of analysis (CoA) for each standard and an example calibration curve with analytical conditions.
The standards need to be weighed before use. Suitable for calibrations in aqueous solutions and DMSO.
Kit contains 10 different pullulan standards, each 100 mg, with Mp ranging from ~350 to ~700,000.

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCM6880
BCCL0102
BCCK1823
BCCJ8040
BCCJ3796

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Quantitative characterization of membrane formation process of alginate?chitosan microcapsules by GPC
Yu W, et al.
Journal of Membrane Science, 346, 296-301 (2010)
Mélanie Lavergne et al.
Macromolecular bioscience, 12(7), 901-910 (2012-06-15)
Human ECFCs contribute to vascular repair. For this reason, they are considered as valuable cell therapy products in ischemic diseases. Porous scaffolds are prepared that are composed of natural polysaccharides, pullulan and dextran, by chemical crosslinking without use of organic
Rahul Garhwal et al.
Investigative ophthalmology & visual science, 53(3), 1341-1352 (2012-01-24)
To formulate conventional contact lenses that incorporate nanosphere-encapsulated antibiotic and demonstrate that the lenses provide for sustained antibacterial activity. A copolymer composed of pullulan and polycaprolactone (PCL) was used to synthesize core-shell nanospheres that encapsulated ciprofloxacin. Bactericidal activity of the
Pei-Hong Miao et al.
Die Pharmazie, 67(3), 229-232 (2012-04-26)
Current efforts had been made to undertake a three-dimensional (3-D) reverse transfection of bone marrow-derived mesenchymal stem cells (BM-MSCs) in PLGA scaffolds. As a kind of multipotent stem cells, BM-MSCs show great potential and tremendous capacity in the gene transfection
Xiaojun Tao et al.
PloS one, 7(11), e49304-e49304 (2012-11-21)
Nanoparticle (NP) compositions such as hydrophobicity and surface charge are vital to determine the presence and amount of human serum albumin (HSA) binding. The HSA binding influences drug release, biocompatibility, biodistribution, and intercellular trafficking of nanoparticles (NPs). Here, we prepared
View All Related Papers

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Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is specification for GPC pullulan standards. Pullulan standard with Mp 22000Da supplied in different lot can vary how much % from 22000Da

    1 answer

    1. This product is intended for use as a calibration standard for GPC/SEC/GFC systems. While there is not specification for the Mp values, these are reported on the lot specific Certificate of Analysis. The lowest value reported for the Mp 220000Da is 19.4000Da. Please see the link below to review a sample or lot specific Certificate:

      https://www.sigmaaldrich.com/product/sial/96351#product-documentation

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