00185

Acetamide Nutrient Broth

suitable for microbiology, NutriSelect^® Basic

• MDL number: MFCD00239573
• UNSPSC Code: 41171607
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 100
• product line: BioChemika
• form: powder
• shelf life: limited shelf life, expiry date on the label
• manufacturer/tradename: NutriSelect^® Basic
• technique(s): microbe id | utilization test: suitable
• final pH: 7.0±0.2 (25 °C)
• application(s): bioburden testing; environmental; food and beverages; water monitoring; microbiology
• suitability: selective by defined nutrients for Pseudomonas spp.

Description

Application

Synthetic medium to detect microbial utilization of acetamide. Corresponds to the suggestions of the "Deutsches Institut für Normung (DIN)" and the test procedures set out in §35 LMBG for the examination of water for Pseudomonas aeruginosa.

Components

Composition

Part A (Cat. No. 62192-109.4-KC)
Magnesium sulfate 0.158g/L
Sodium chloride 0.2g/L
Sodium molybdate 0.005g/L
Ferrous sulfate 0.0005g/L
Dipotassium hydrogen phosphate 0.2g/L

Part B (Cat. No. 00160-390.6G-KC)
Acetamide 2.0g/L

Preparation Note

Directions:
Dissolve 0.56 g Acetamide Nutrient Broth Part A and 2 g Acetamide in 950 ml distilled water. Check the pH and if necessary, adjust. Make up to 1 litre and pour into tubes. Autoclave at 121°C for 15 minutes.

Technique:
Medium is inoculated with a couple off loops from the culture to be assayed and it is incubated at 32-35°C for 24-48 hours before going to the isolation medium.
To confirm Ps. aeruginosa, inoculate a loop of culture in Asparagine Proline Broth (Cat. No. 17129) and incubate at 35-37°C for 24 hours. After this period, pour 1-2 drops of Nessler′s Reagent
(Cat. No. 72190) on to the culture and verify ammonia production: a change to yellow will mean ammonia production positive and therefore, Ps. aeruginosa presence.

Other Notes

Decomposition of acetamide as taxonomic marker for some species of the genus Pseudomonas; Comparative studies on the enrichment of P. aeruginosa

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08
• Signal Word: Warning
• Hazard Statements: H351
• Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves