Skip to Content
Merck
All Photos(1)

Documents

04942078001

Roche

Dispase® II (neutral protease, grade II)

lyophilized, from bacterial, Roche, pkg of 5 × 1 g

Synonym(s):

dissociating enzyme, neutral protease, tissue dissociating

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Enzyme Commission number:
3.4.24.4 (BRENDA, IUBMB)
UNSPSC Code:
12352204
NACRES:
NA.28

biological source

bacterial

Quality Level

100

sterility

non-sterile

form

lyophilized

specific activity

≥0.8 units/mg protein (37 °C, casein as substrate, pH 7.5)

packaging

pkg of 5 × 1 g

manufacturer/tradename

Roche

optimum pH

6.0-8.5

application(s)

sample preparation

Related Categories

General description

Dispase is a neutral, rapid-acting, gentle protease that separates intact epidermis from dermis. It also separates cultured intact epithelial cells from the substratum. It cleaves the basement membrane zone and leaves the epithelial cells intact.

Specificity

Dispase is a nonspecific metalloprotease.

Application

Dispase® is used for the preparation of cells from a wide variety of different tissues and organs. The enzyme has proven to be a rapid, effective, yet gentle agent for the disruption of extracellular matrix of tissues for releasing single cells for cell culture (primary cell culture) or harvesting cells already in culture to transfer to new substrate (secondary culture). Furthermore, it is used to prevent unwanted clumping of cells cultured in suspension.

Features and Benefits

  • Rapid, effective, yet gentle agent that liberates cells with minimal cell damage
  • Maintains cell membrane integrity
  • Non-mammalian source - free of mycoplasma and animal virus contamination
  • Extremely stable to influences of temperature, pH, and interference by serum components
  • Easily inactivated by chelating agents or by dilution
  • Delivers higher activity and convenience

Unit Definition

One unit is the enzyme activity which liberates Folin-positive amino acids and peptides corresponding to 1 μmol (181 μg) tyrosine in 1 minute under assay conditions (pH 7.5, +37 °C).
A practical comparison of Roche units of Dispase® II with those cited in the Japanese literature (where Dispase® concentrations of 1,000 to 2,000 units/ml are not uncommon) suggests that one Roche unit equals approximately 600 Japanese units of dispase.
One unit of Roche Applied Science Dispase® equals 181 protease units (PU) measured as release of amino acids equivalent to 1 μg tyrosine per min and ml at pH 7.5 and +37 °C.

Preparation Note

Activator: Optimal Ca2+ concentration is 2 mM. The enzyme preparations contain enough Ca2+ for optimal activity.
Inhibitors: EDTA, EGTA, Hg2+, other heavy metals. Dispase is not inhibited by serum.
Working concentration: 0.6 to 2.4 U/ml
Working solution: Preparation of stock and working solutions:

To produce a 10 mg/ml stock solution, dissolve the lyophilized Dispase® II enzyme in HEPES-buffered saline (50 mM HEPES/KOH pH 7.4, 150 mM NaCl). To produce the working solution, dilute the above stock solution with the culture medium for the isolated cells, at a final concentration of 0.6 to 2.4 U/ml. Note that concentrations higher than 2.4 U/ml are not recommended. For best results, filter the working solution using a 0.22 μm filter membrane.
Storage conditions (working solution): -15 to -25 °C
The reconstituted stock solution is stable at 2 to 8 °C for 2 weeks. For storage up to 2 months the stock solution should be frozen in aliquots. Avoid repeated freezing and thawing! The working solution diluted with PBS is stable at 2 to 8 °C for 3 days.

Storage and Stability

Store at 2 to 8 °C. (Store dry!)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Dispase is a registered trademark of Godo Shusei Co., Ltd.

Pictograms

Exclamation markHealth hazard
GHS07,GHS08

Signal Word

Danger

Hazard Statements

H315 - H319 - H334 - H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Nicholas R Hum et al.
Cancers, 12(3) (2020-03-19)
Current pre-clinical models of cancer fail to recapitulate the cancer cell behavior in primary tumors primarily because of the lack of a deeper understanding of the effects that the microenvironment has on cancer cell phenotype. Transcriptomic profiling of 4T1 murine
Zhonghou Wang et al.
ACS pharmacology & translational science, 3(4), 749-758 (2020-08-22)
As a result of the COVID-19 pandemic, evidence revealed that SARS-CoV-2 infection caused taste loss at a rate higher than that of influenza. ACE2, the entry receptor of SARS-CoV-2, has been identified in the oral epithelium; however, it is unclear
Youjin Na et al.
Oncogene, 40(5), 951-963 (2020-12-10)
MicroRNAs (miRs) are small non-coding RNAs that can have large impacts on oncogenic pathways. Possible functions of dysregulated miRs have not been studied in neurofibromatosis type 1 (NF1) plexiform neurofibromas (PNFs). In PNFs, Schwann cells (SCs) have biallelic NF1 mutations
Johanna Tukler Henriksson et al.
Investigative ophthalmology & visual science, 56(8), 4186-4197 (2015-07-02)
To investigate the effects of IL-13 on goblet cell proliferation, differentiation, and expression of mucin and immunomodulatory genes. Explants were excised from the conjunctiva of young C57BL/6 mice. Cultures received 200 μL per week of either Keratinocyte media (KSFM) or
Chen Chen et al.
Bone research, 8, 7-7 (2020-03-05)
Lumbar degenerative disc diseases cause low back pain (LBP). The maintenance of the height and stability of the intervertebral disc (IVD) space is an effective treatment for LBP. The following study evaluated the effects of fibroblast injection on intervertebral disc
View All Related Papers

Related Content

Tissue Dissociation Guide: Collagenase, Dispase, and Liberase Enzyme Types

Collagenase Guide.Collagenases, enzymes that break down the native collagen that holds animal tissues together, are made by a variety of microorganisms and by many different animal cells.

Tissue Dissociation Guide: Collagenase, Dispase, and Liberase Enzyme Types

Collagenase Guide.Collagenases, enzymes that break down the native collagen that holds animal tissues together, are made by a variety of microorganisms and by many different animal cells.

Tissue Dissociation Guide: Collagenase, Dispase, and Liberase Enzyme Types

Collagenase Guide.Collagenases, enzymes that break down the native collagen that holds animal tissues together, are made by a variety of microorganisms and by many different animal cells.

Tissue Dissociation Guide: Collagenase, Dispase, and Liberase Enzyme Types

Collagenase Guide.Collagenases, enzymes that break down the native collagen that holds animal tissues together, are made by a variety of microorganisms and by many different animal cells.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service