This product is presented as a 40 mM solution in distilled water, at pH 4.1 ± 0.1, and is ready-to-use. The recommended volume is 1 ml for a 24-well plate and 0.5 ml for a 48-well plate.
TMS-008
Alizarin-Red Staining Solution
Alizarin red is a commonly used stain to identify calcium containing osteocytes in differentiated culture of both human and rodent mesenchymal stem cells (MSCs).
Synonym(s):
Alizarin Red S, Alizarin Red S Stain, Alizarin Red Stain
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Pricing and availability is not currently available.
Recommended Products
Quality Level
concentration
40 mM in distilled water
technique(s)
cell based assay: suitable
cell culture | stem cell: suitable
cell differentiation: suitable
Related Categories
General description
Alizarin Red, an anthraquinone derivative, may be used to identify calcium in tissue sections and cultured cells in vitro. The reaction is not strictly specific for calcium, since magnesium, manganese, barium, strontium, and iron may interfere, but these elements usually do not occur in sufficient concentration to interfere with the staining. Calcium forms an Alizarin Red S-calcium complex in a chelation process, and the end product is a bright red stain. Alizarin red is a commonly used stain to identify calcium containing osteocyte in differentiated culture of both human and rodent mesenchymal stem cells.
Application
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Osteobiology
Arthritis
Osteobiology
Arthritis
Quality
Solution is red with no particulates present. Solution is suitable for endpoint cell culture assays.
Physical form
Product is presented as a 40mM solution at pH 4.1 +/- 0.1.
Storage and Stability
Store at 2-8ºC for up to 4 months from date of receipt. If Alizarin-red solution forms a precipitate, remove particulates by passage through a 0.22 or 0.45-micron filter.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Usually it is prepare a 2% solution for cell culture uses. This solution is used directly without any previous dilution?
1 answer-
Helpful?
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What difference between products TMS-008 Alizarin-Red Staining Solution and 2003999 Alizarin Red Solution?
1 answer-
Products TMS-008 and 2003999 are similar products. Product TMS-008 is presented as a 40mM solution at pH 4.1 +/- 0.1.
Product 2003999 is also a 40mM solution with pH range 4.1 +/- 0.1. It is also a kit component of Product ECM815.
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Dear Sir/Madam, Hope you are well. We already ordered ARS from your company. But we unfortunately lost the protocol inside the box. I was wondering if yo can email us the protocol. Best Regards, Ebrahim
1 answer-
Regarding your inquiry about TMS-008-C Alizarin-Red Staining Solution, there is currently no protocol available on sigmaaldrich.com for staining cells with TMS-008. The following information does appear on the emdmillipore.com website:
Product is presented as a 40mM solution at pH 4.1 +/- 0.1.As the molecular weight is 342.26, the percentage of Alizarin Red should be 1.4%. Most of the common histology textbooks generally recommend using a 2% concentration of Alizarin Red. But as in many histology procedures, the exact percentage is generally not critical for acceptable staining. It would appear the developers of the solution thought they preferred a concentration of 1.4%. This is typical of many histological staining procedures. Methods can and do vary from source to source.
As to a protocol, protocols for most histology textbooks should be acceptable for use. In general, tissue should not be fixed in fixative that contain calcium. Fixatives commonly used as 10% Neutral Buffered Formalin or alcoholic formalin. Once the slides have been prepared, hydrate sections in 50% alcohol. Then rinse briefly with distilled water. Incubate the slides in the Alizarin Red solution anywhere from 30 second to 5 minutes or more. Check for stain development every 30 seconds under a microscope until a red lake forms. The lake should not be too diffuse and on the heavy side. Then remove the solution by shaking off the excess and blot dry. Flood slides with acetone for about 20 seconds. Use a 50:50 mixture of acetone and xylene to differentiate. Clear in xylene. If possible, mount with a synthetic mounting media.
The solution does not have great stability. The website further states: Store at 2-8ºC for up to 4 months from date of receipt. If Alizarin-red solution forms a precipitate, remove particulates by passage through a 0.22 or 0.45-micron filter.
This product is included in some of our kits, such as ECM815 or ECM810. Here are the user guides for the kit ECM815 and ECM810, which contains information about the Alizarin red solution protocol:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/190/283/ecm810man.pdf
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/324/119/ecm815.pdfYou can also find application information for Alizarin Red at the following links:
https://www.sigmaaldrich.com/technical-documents/protocol/cell-culture-and-cell-culture-analysis/stem-cell-culture/mesenchymal-stem-cell-culture-protocolsHelpful?
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