SCT247

BioTracker MultiTASQ G-Quadruplex (G4) Cell Probe

• Synonym(s): G quadruplex probe, G4 ligand, G4 probe, G4 tetrad probe, Live Cell Imaging Probe, click chemistry probe
• UNSPSC Code: 41106514
• NACRES: NA.52

Properties

• packaging: vial of 1 mg
• Quality Level: 100
• manufacturer/tradename: Millipore
• growth mode: N/A
• technique(s): DNA sequencing: suitable; RNA extraction: suitable; nucleic acid detection: suitable
• detection method: fluorometric
• shipped in: dry ice
• storage temp.: −20°C

Description

Application

G-quadruplexes are four-stranded structures consisting of stacked G tetrads (G4) formed within select guanine-rich nucleic acid sequences. G-quadruplexes are enriched in open chromatin regions but are also present in the nucleolus and cytoplasm. The formation of G4 structures is dynamic and cell-type specific, suggesting interaction with transcription factors and other proteins. G-quadruplexes have therefore attracted acute interest in their possible contribution in transcriptional regulation.

MultiTASQ was designed utilizing the template-assembled synthetic G-quartets (TASQ) concept as biomimetic ligand for G-quadruplexes, demonstrating high specificity for G4. MultiTASQ is water-soluble and exhibits a high degree of intracellular accessibility. MultiTASQ detects ribosomal RNA-G4 associated with perinuclear rough ER regions to a greater degree compared to BG4 antibody. MultiTASQ is an alkynylated probe that allows for background-free detection via click chemistry and is suitable for G4 capture chemi-precipitation and click imaging applications.

References
1. Nat. Chem. 2021, 13(7): 626-633
2. ACS Chem Biol. 2021, 16(5): 905-914

Purity: > 98% confirmed by HPLC, HNMR, LC-MS and elemental analysis.

Features and Benefits

MultiTASQ detects ribosomal RNA-G4 associated with perinuclear rough ER regions to a greater degree compared to BG4 antibody. MultiTASQ is an alkynylated probe that allows for background-free detection via click chemistry and is suitable for G4 capture chemi-precipitation and click imaging applications. MultiTASQ is compatible with live-cell incubation. The BioTracker MultiTASQ G4 cell probe is a uniquely designed, versatile tool for G4 detection and analysis.

Physical form

Lyophilized. White solid.

Storage and Stability

Store BioTracker BioCyTASQ G-quadruplex G4 cell probe at -20°C, desiccated and protected from light

Other Notes

Fluorescent detection of nucleic acid G4 quadruplexes in cells and tissues using click chemistry

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable