Skip to Content
Merck
All Photos(1)

Key Documents

MABF125

Sigma-Aldrich

Anti-TAP1 Antibody, clone mAb 148.3

clone mAb 148.3, from mouse

Synonym(s):

Antigen peptide transporter 1, APT1, ATP-binding cassette sub-family B member 2, Peptide supply factor 1, Peptide transporter PSF1, PSF-1, Peptide transporter TAP1, Peptide transporter involved in antigen processing 1, Really interesting new gene 4 prote

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

mAb 148.3, monoclonal

species reactivity

human

technique(s)

activity assay: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TAP1(6890)

General description

Peptide transporter TAP1 is also called Antigen peptide transporter 1 (APT1), ATP-binding cassette sub-family B member 2 (ABCB2), Peptide supply factor 1 (PSF1), Peptide transporter PSF1 (PSF-1), Peptide transporter involved in antigen processing 1, and Really interesting new gene 4 protein (RING4). TAP1 is involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules as well as MHC class I folding. TAP1 is inhibited herpes simplex virus ICP47 protein, human cytomegalovirus US6 glycoprotein, human adenovirus E3-19K glycoprotein, and is down-regulated by human Epstein-Barr virus vIL-10 protein. TAP1 mutations can be a cause of Bare lymphocyte syndrome 1 (BLS1).

Immunogen

Epitope: This antibody recognizes the ADAPE amino acid residues within the CYWAMVQAPADAPE sequence of human TAP1 (located at the C-terminus).
Linear peptide corresponding to the CYWAMVQAPADAPE sequence of human TAP1.

Application

Detect Antigen peptide transporter 1 using this mouse monoclonal antibody, Anti-TAP1 Antibody, clone mAb 148.3 validated for use in western blotting, IP, Immunofluorescence & Activity Assay.
Western Blotting Analysis: A representative lot from an independent laboratory detected TAP1 in transiently transfected HeLa cells (Hulpke, S., et al. (2012). FASEB J. 26(12):5071-5080.).

Western Blotting Analysis: A representative lot from an independent laboratory detected TAP1 in microsomes of baculovirus-infected SF9 cells, which express wild type or select mutations of TAP1 (Chen, M., et al. (2004). J Biol Chem. 279(44):46073-46081.).

Western Blotting Analysis: A representative lot from an independent laboratory detected TAP1 in SF9 cells infected with recombinant baculovirus containing TAP1 gene constructs (Meyer, T. H., et al. (1994). FEBS Lett. 351(3):443-447.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected TAP1 in HeLa cells contransfected with wild type TAP1 and TAP2 (Hulpke, S., et al. (2012). Cell Mol Life Sci. 69(19):3317-3327.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected TAP1 in SF9 cells infected with rBV-TAP1/rBV-TAP2 (Meyer, T. H., et al. (1994). FEBS Lett. 351(3):443-447.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated TAP1 from SF9 cells infected with rBV-TAP1/rBV-TAP2 (Meyer, T. H., et al. (1994). FEBS Lett. 351(3):443-447.).

Activity Assay Analysis: This antibody inhibits TAP-specific peptide transport (Plewnia, G., et al. (2007). J Mol Biol. 369(1):95-107.).

Quality

Evaluated by Western Blotting in untreated and Interferon-gamma (IFN-g) treated HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected TAP1 in 10 µg of Interferon-gamma (IFN-g) treated HeLa cell lysate.

Target description

~65 kDa observed. Uniprot describes a molecular weight of ~87 kDa However, this protein may be observed at ~65 kDa (Koch, J., et al. (2006). FEBS Lett. 580(17):4091-4096.).

Physical form

Format: Purified
Purified mouse monoclonal IgG1κ supernatant in buffer containing PBS without preservatives.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Man Huang et al.
Scientific reports, 6, 33612-33612 (2016-09-17)
HLA class I (HLA-I) transgenic mice have proven to be useful models for studying human MHC-related immune responses over the last two decades. However, differences in the processing and presentation machinery between humans and mice may have profound effects on
Ilse Dingjan et al.
European journal of cell biology, 96(7), 705-714 (2017-07-10)
Cross-presentation of foreign antigen in major histocompatibility complex (MHC) class I by dendritic cells (DCs) requires activation of the NADPH-oxidase NOX2 complex. We recently showed that NOX2 is recruited to phagosomes by the SNARE protein VAMP8 where NOX2-produced reactive oxygen
Brendan L C Kinney et al.
Cancer immunology, immunotherapy : CII, 73(1), 10-10 (2024-01-17)
The antigen processing machinery (APM) components needed for a tumor cell to present an antigen to a T cell are expressed at low levels in solid tumors, constituting an important mechanism of immune escape. More than most other solid tumors
Devin Dersh et al.
Immunity, 54(1), 116-131 (2020-12-04)
Tumors frequently subvert major histocompatibility complex class I (MHC-I) peptide presentation to evade CD8+ T cell immunosurveillance, though how this is accomplished is not always well defined. To identify the global regulatory networks controlling antigen presentation, we employed genome-wide screening in

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service