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MAB3878

Sigma-Aldrich

Anti-MyoD1 Antibody

clone 5.2F, Chemicon®, from mouse

Synonym(s):

Anti-MYF3, Anti-MYOD, Anti-MYODRIF, Anti-PUM, Anti-bHLHc1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5.2F, monoclonal

species reactivity

rat, mouse, chicken, human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2a

NCBI accession no.

NM_002478.4

UniProt accession no.

P15172

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MYOD1(4654)

Specificity

Recognizes MyoD1. The epitope is localized to amino acids 3-56 in the N-terminus of mouse MyoD1 protein.

Immunogen

Recombinant protein from mouse MyoD1.

Application

Anti-MyoD1 Antibody is a high quality Mouse Monoclonal Antibody for the detection of MyoD1 & has been validated in ICC, IHC, IP & WB.
Western blot: 1 μg/mL. Reacts with the ~45 kD protein.

Immunohistochemistry on frozen and formalin-fixed, paraffin embedded tissue: 2-4 μg/mL.

Immunocytochemistry

Immunoprecipitation: 2 μg/mg of protein lysate

Optimal working dilutions must be determined by end user.

Target description

~ 45 kDa

Physical form

Format: Purified

Analysis Note

Control
Fetal Muscle, Rhabdomyosarcoma

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A recurrent neomorphic mutation in MYOD1 defines a clinically aggressive subset of embryonal rhabdomyosarcoma associated with PI3K-AKT pathway mutations.
Kohsaka, S; Shukla, N; Ameur, N; Ito, T; Ng, CK; Wang, L; Lim, D; Marchetti, A; Viale et al.
Nature Genetics null
Mark A Gillespie et al.
The Journal of cell biology, 187(7), 991-1005 (2009-12-23)
The mitogen-activated protein kinase p38-gamma is highly expressed in skeletal muscle and is associated with the dystrophin glycoprotein complex; however, its function remains unclear. After induced damage, muscle in mice lacking p38-gamma generated significantly fewer myofibers than wild-type muscle. Notably
Francesca Megiorni et al.
Journal of hematology & oncology, 10(1), 161-161 (2017-10-08)
EPH (erythropoietin-producing hepatocellular) receptors are clinically relevant targets in several malignancies. This report describes the effects of GLPG1790, a new potent pan-EPH inhibitor, in human embryonal rhabdomyosarcoma (ERMS) cell lines. EPH-A2 and Ephrin-A1 mRNA expression was quantified by real-time PCR
In situ mechanotransduction via vinculin regulates stem cell differentiation.
Holle, AW; Tang, X; Vijayraghavan, D; Vincent, LG; Fuhrmann, A; Choi, YS; del Alamo, JC; Engler, AJ
Stem Cells null
Junmin Lee et al.
Biomaterials, 69, 174-183 (2015-08-19)
Cells sense and transduce the chemical and mechanical properties of their microenvironment through cell surface integrin receptors. Traction stress exerted by cells on the extracellular matrix mediates focal adhesion stabilization and regulation of the cytoskeleton for directing biological activity. Understanding
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