MAB347
Anti-Growth Associated Protein 43 Antibody, clone 9-1E12
clone 9-1E12, Chemicon®, from mouse
Synonym(s):
Neuromodulin, B-50 Protein, Growth Associated Protein 43, neuromodulin
About This Item
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
9-1E12, monoclonal
species reactivity
feline, rat, hamster
species reactivity (predicted by homology)
human
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... GAP43(2596)
General description
Specificity
Immunogen
Application
0.1-0.5 µg/mL of a previous lot on 4% paraformaldehyde perfused and fixed rat cerebellum and cerebrum tissue. The addition of 0.01-0.1% Triton X-100 to incubation buffer will increase cellular permeability.
Immunoprecipitation:
A previous lot of this antibody was used in immunoprecipitation.
5-10µg/mL in membrane preparations from 16-18 day embryonic cortical neuronal cultures.
Western Blot Analysis:
MAB347 will detect a single 43-48 kD band in western blots of membrane fractions of growing neurons.
Optimal working dilutions must be determined by the end user.
Neuroscience
Neuroregenerative Medicine
Quality
Western Blot Analysis:
1:1000 dilution of this lot detected GAP-43/B-50 on 10 μg of rat brain lysates.
Target description
Physical form
Storage and Stability
Analysis Note
Rat DRG tissue that has been subjected to a spinal nerve ligation and cultured neurons.
Other Notes
Legal Information
Disclaimer
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recommended
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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