The amount of material in the vial will change from lot to lot. The minimum activity for this enzyme is 300 Units per milligram solid. Upon review of the last 5 lots, the average amount of product in the 1000U vial is 2.5 mg. Activities have ranged from 326 - 530 units per milligram.
189724
Ascorbate Oxidase, Cucurbita sp.
Native ascorbate oxidase from Cucurbita species. Catalyzes the oxidation of ascorbioxidation of ascorbic acid to dehydroascorbic acid. Suitable for eliminating interference of ascorbic acid in clinical assays and for determination of ascorbic acid.
Synonym(s):
Ascorbate Oxidase, Cucurbita sp.
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.54
Pricing and availability is not currently available.
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Quality Level
form
lyophilized
specific activity
≥1500 U/mg
≥300 units/mg material
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated (hygroscopic)
shipped in
ambient
storage temp.
−20°C
General description
Native ascorbate oxidase from Cucurbita species. A multi-copper-containing oxidase that catalyzes oxidation of ascorbic acid to dehydroascorbic acid. AO catalyses the four-electron reduction of dioxygen to water with concominant one-electron oxidation of the reducing organic substrate. Suitable for eliminating interference of ascorbic acid in clinical assays and for determination of ascorbic acid. Inhibited by azide, dithiocarbamate, and thiocyanate.
Caution
enzyme will denature below pH 4.0.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will oxidize 1.0 µmol ascorbic acid per min at 37°C, pH 5.6.
Reconstitution
Following reconstitution refrigerate (4°C). This Stock solutions are stable for up to 3 weeks at 4°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Approximately how many mg of protein is in the 1000U vial of SKU 189724-1000U? For my purposes, I am not interested in enzymatic activity, only protein mass. I need more than 2mg up to ~10mg of this protein.
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