05-421
Anti-PP2A Antibody, C subunit, clone 1D6
clone 1D6, Upstate®, from mouse
Synonym(s):
Replication protein C, protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform, protein phosphatase 2, catalytic subunit, alpha isoform, serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform
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All Photos(4)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
1D6, monoclonal
species reactivity
Saccharomyces cerevisiae, bovine, rabbit, rat, Xenopus, mouse, human
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... PPP2CA(5515)
General description
Type 2 protein serine/threonine phosphatase (PP2A) is a trimer composed of a catalytic C subunit and two regulatory (A and B) subunits; many isoforms for the A and B subunits have been found. PP2A has a high specific activity against the α subunit of phosphorylase kinase and is insensitive to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and by its high sensitivity to inhibition by okadaic acid. It is also thought to be the principal downmodulator of serine/threonine kinase cascades. PP2B requires Ca2+/calmodulin for activity, and is an activator of NFAT transcription factors. PP2C requires Mg2+, but is insensitive to okadaic acid, microcystin LR, or the calmodulin inhibitor trifluoperazine.
Specificity
This antibody recognizes PP2A catalytic subunit, Mr 36 kDa.
Immunogen
A 16 residue synthetic peptide (C-RGEPHVTRRTPDYFL) corresponding to amino acids 295-309 of the 36 kDa catalytic subunit of human protein phosphatase 2A (PP2A). Clone 1D6.
Epitope: a.a. 295-309
Application
Detect PP2A using this Anti-PP2A Antibody, C subunit, clone 1D6 validated for use in IC, IP & WB.
Immunoprecipitation:
4 µg of a previous lot immunoprecipitated PP2A from 500 µg of A431 RIPA lysate. Note: This antibody has been reported not to block phosphatase activity.
Immunocytochemistry:
5-10 µg/mL of a previous lot of this antibody showed positive immunostaining for PP2A in A431 cells fixed for 10 minutes with 4% paraformaldehyde in PBS.
4 µg of a previous lot immunoprecipitated PP2A from 500 µg of A431 RIPA lysate. Note: This antibody has been reported not to block phosphatase activity.
Immunocytochemistry:
5-10 µg/mL of a previous lot of this antibody showed positive immunostaining for PP2A in A431 cells fixed for 10 minutes with 4% paraformaldehyde in PBS.
Research Category
Signaling
Neuroscience
Signaling
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurodegenerative Diseases
Neurotransmitters & Receptors
Neurodegenerative Diseases
Quality
Routinely evaluated by western blot on human A431 or murine 3T3/NIH RIPA cell lysates.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected PP2A in human A431 and previously in murine 3T3 RIPA cell lysates.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected PP2A in human A431 and previously in murine 3T3 RIPA cell lysates.
Target description
36 kDa
Physical form
Format: Purified
Protein A chromatography
Purified mouse monoclonal IgG2bκ in buffer containing 0.1M Tris-Glycine, pH 7.4, 0.15 M, NaCl, and 0.05% sodium azide. Frozen solution.
Storage and Stability
Stable for 1 year at -20ºC from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
H Wei et al.
The Journal of biological chemistry, 276(2), 1570-1577 (2000-10-20)
Protein phosphatase 2A (PP2A) is an essential eukaryotic serine/threonine phosphatase known to play important roles in cell cycle regulation. Association of different B-type targeting subunits with the heterodimeric core (A/C) enzyme is known to be an important mechanism of regulating
Circumventing embryonic lethality with Lcmt1 deficiency: generation of hypomorphic Lcmt1 mice with reduced protein phosphatase 2A methyltransferase expression and defects in insulin signaling.
MacKay, KB; Tu, Y; Young, SG; Clarke, SG
Testing null
Identification of protein phosphatase 1 as a mitotic lamin phosphatase
Thompson, L. J., et al
The Journal of Biological Chemistry, 272, 29693-29697 (1997)
Greatwall-phosphorylated Endosulfine is both an inhibitor and a substrate of PP2A-B55 heterotrimers.
Williams, BC; Filter, JJ; Blake-Hodek, KA; Wadzinski, BE; Fuda, NJ; Shalloway, D; Goldberg, ML
eLife null
Characterization of the PP2A alpha gene mutation in okadaic acid-resistant variants of CHO-K1 cells
Shima, H, et al
Proceedings of the National Academy of Sciences of the USA, 91, 9267-9271 (1994)
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