123072

Luminol

97%

• Synonym(s): 3-Aminophthalhydrazide, 5-Amino-2,3-dihydro-1,4-phthalazinedione
• Empirical Formula (Hill Notation): C₈H₇N₃O₂
• CAS Number: 521-31-3
• Molecular Weight: 177.16
• Beilstein: 383929
• EC Number: 208-309-4
• MDL number: MFCD00006890
• UNSPSC Code: 12352103
• PubChem Substance ID: 24847519
• NACRES: NA.23

Properties

• Assay: 97%
• mp: >300 °C (lit.)
• SMILES string: Nc1cccc2C(=O)NNC(=O)c12
• InChI: 1S/C8H7N3O2/c9-5-3-1-2-4-6(5)8(13)11-10-7(4)12/h1-3H,9H2,(H,10,12)(H,11,13)
• InChI key: HWYHZTIRURJOHG-UHFFFAOYSA-N

Description

General description

Luminol is a very efficient and commonly used chemiluminiscent compound in condensed phase. When basic solutions of luminol containing oxygen are treated with an oxidizing agent, light is produced. Oxidising agents capable of initiating this particular reaction are Nitrogen dioxide, PAN, hydrogen peroxide, ozone and other atmospheric oxidants.

Application

• As a chemiluminiscent substance. Used to measure opsonic and phagocytic function.
• As a biological sensor. Used to detect the polymorphonuclear leukocytes (PMNL) response in a patient with a myeloperoxidase (MPO) deficiency.
• Used as a forensic test for blood.

Luminol has been used in a protocol to study the immune-related responses in Arabidopsis thaliana via luminol/peroxidase-based assay.

Features and Benefits

• Very effective chemiluminiscent compound.
• Can detect sub-part per billion levels of metal ions with simple and inexpensive instrumentation.

Used for chemiluminescence analysis of, for example, metal cations, blood, and glucococorticoids.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves