Accéder au contenu
Merck

Fast and sensitive DNA hybridization assays using microwave-accelerated metal-enhanced fluorescence.

Biochemical and biophysical research communications (2006-08-08)
Kadir Aslan, Stuart N Malyn, Chris D Geddes
RÉSUMÉ

A new, fast, and sensitive DNA hybridization assay platform based on microwave-accelerated metal-enhanced fluorescence (MAMEF) is presented. Thiolated oligonucleotide anchors were immobilized onto silver nanoparticles on a glass substrate. The hybridization of the complementary fluorescein-labeled DNA target with the surface-bound oligonucleotides was completed within 20 s upon heating with low-power microwaves. In addition, the signal is optically amplified, a consequence of close proximity of the fluorophore to the silvered substrate. In this proof-of-principle methodology, as low as 50 nM of a target DNA was detected, although we envisage far-lower detection limits. Control experiments, where the surface-bound oligonucleotide was omitted, were also performed to determine the extent of non-specific binding. In these studies a significantly reduced non-specific adsorption was found when using microwave heating near to silvered structures as compared to room temperature incubation. These findings suggest that MAMEF could be a most useful alternative to the DNA hybridization assays used today, especially with regard to substantially increasing both the assay rapidity and sensitivity.

MATÉRIAUX
Référence du produit
Marque
Description du produit

Sigma-Aldrich
Acridine Yellow G, Dye content 90 %
Référence
Conditionnement
Disponibilité
Prix
Quantité