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W0138

Sigma-Aldrich

Western Blocker™ Solution

for HRP detection systems

Synonyme(s) :

Western blot blocking solution, blocking buffer

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About This Item

Code UNSPSC :
41105329
Nomenclature NACRES :
NA.25

Niveau de qualité

200

Durée de conservation

≥2 yr

Température de stockage

2-8°C

Catégories apparentées

Description générale

Western Blocker Solution is used to block both PVDF and nitrocellulose membranes after proteins have been transferred onto them through dot blotting or Western blotting; blocking is performed to reduce or eliminate nonspecific binding of primary or secondary antibodies.

Application

Western Blocker Solution has been optimized for detection systems using horseradish peroxidase (HRP) conjugates; it is also compatible with alkaline phosphatase based systems.
Western Blocker Solution has been used for blocking of western blot membranes. It has also been used for dilution of antibodies in western blot.

Caractéristiques et avantages

  • Does not inhibit weak HRP signals, as many milk-based blockers do.
  • Can be used both as a blocking component and as a probing component.
  • Supplied ready-to-use; no preparative step is required.

Informations légales

Western Blocker is a trademark of Sigma-Aldrich Co. LLC

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Lalit P Chandravanshi et al.
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, 34, 60-75 (2014-02-13)
In view of the increasing incidences of arsenic induced health effects and the vulnerability of the developing brain to its toxic effects, studies have been carried out to investigate the mechanism of arsenic induced cholinergic alterations and understand if such
In vitro differentiation of human bone marrow mesenchymal stem cells into hepatocyte-like cells.
Pournasr B, et al.
Archives of Iranian Medicine, 14, 244-249 (2011)
Massimo Conese et al.
International journal of molecular sciences, 21(12) (2020-06-26)
Recently, insulin-like growth factor binding protein 6 (IGFBP-6) has been shown to play a putative role in the immune system, as monocyte-derived dendritic cells (Mo-DCs) are stimulated by hyperthermia to express IGFBP-6 at both the mRNA and protein levels. However
A N Meleshko et al.
Experimental oncology, 35(1), 8-14 (2013-03-27)
Idiotype, the unique part of immunoglobulin molecule expressed on the surface of B-cells, represents a specific antigen for vaccination against lymphoma. We have developed a rapid method for immunoglobulin variable fragments cloning, assembling and expression of recombinant idiotype protein in
Ayça Fulya Üstüntanır Dede et al.
Gene, 748, 144697-144697 (2020-04-24)
Artificial chromosomes were previously generated for use in bacteria, protists, yeast and human cells. A Tetrahymena thermophila artificial chromosome could serve as a versatile platform to study diverse aspects of Tetrahymena biology and beyond. Here, we placed a C3-type rDNA
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