Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

View All Documentation

T7254

Sigma-Aldrich

Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride

powder, ≥96% (TLC)

Synonyme(s) :

(3S)-1-Chloro-3-tosylamido-7-amino-2-heptanone hydrochloride, (3S)-7-Amino-1-chloro-3-tosylamino-2-heptanone hydrochloride, TLCK, Tosyl-L-lysyl-chloromethane hydrochloride

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Formule empirique (notation de Hill):
C14H21ClN2O3S · HCl
Numéro CAS:
4272-74-6
Poids moléculaire :
369.31
Numéro Beilstein :
7106867
Numéro CE :
224-266-4
Numéro MDL:
MFCD00065395
Code UNSPSC :
12352202
ID de substance PubChem :
24278752
Nomenclature NACRES :
NA.77

product name

Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride, ≥96% (TLC), powder

Source biologique

synthetic (organic)

Niveau de qualité

200

Pureté

≥96% (TLC)

Forme

powder

Puissance

10-100 μM effective concentration

Solubilité

H2O: 50 mg/mL

Température de stockage

−20°C

Chaîne SMILES 

Cl[H].Cc1ccc(cc1)S(=O)(=O)N[C@@H](CCCCN)C(=O)CCl

InChI

1S/C14H21ClN2O3S.ClH/c1-11-5-7-12(8-6-11)21(19,20)17-13(14(18)10-15)4-2-3-9-16;/h5-8,13,17H,2-4,9-10,16H2,1H3;1H/t13-;/m0./s1

Clé InChI

YFCUZWYIPBUQBD-ZOWNYOTGSA-N

Informations sur le gène

human ... NFKB1(4790) , NFKB2(4791)

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Application

Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride has been used:
  • in chymotrypsin purification to prevent binding of trypsins to the affinity support by inhibiting them in the crude extract
  • as a protease inhibitor in tissue and cell homogenization
  • as a trypsin inhibitor to determine the specific protease activity levels

Actions biochimiques/physiologiques

Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride (TLCK) blocks the lipopolysaccharide (LPS)- or cytokine-induced activation of nuclear factor κB (NF-κB), which, in turn, blocks the induction of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) transcription. Blocks activation of pp70s6k by all mitogens. Blocks apoptosis in cell lines by inhibiting the processing of caspases in some cell lines and to some stimuli.

Conditionnement

Bottomless glass bottle. Contents are inside inserted fused cone.

Attention

Stable at least two years when stored desiccated at −20°C.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Gloves

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Les clients ont également consulté

Slide 1 of 5

1 of 5

Collagénase/dispase® lyophilized, non-sterile, optimum pH 7.0-8.0

Roche

COLLDISP-RO

Collagénase/dispase®

Collagénase D from Clostridium histolyticum

Roche

COLLD-RO

Collagénase D

E-64 protease inhibitor

Sigma-Aldrich

E3132

E-64

3,4-Dichloroisocoumarin serine protease inhibitor

Sigma-Aldrich

D7910

3,4-Dichloroisocoumarin

AEBSF, Hydrochloride AEBSF, CAS 30827-99-7, is a specific irreversible inhibitor of chymotrypsin, kallikrein, plasmin, thrombin, trypsin, and related thrombolytic enzymes. A nontoxic alternative to PMSF and DFP.

Sigma-Aldrich

101500

AEBSF, Hydrochloride

F D'Acquisto et al.
FEBS letters, 418(1-2), 175-178 (1997-12-31)
We investigated the involvement of NF-kappaB in the regulation of COX-2 protein expression and prostaglandin production in LPS-stimulated J774 macrophages. Incubation of J774 cells with LPS (1 microg/ml) for 24 h caused an increase of COX-2 protein expression and accumulation
Feces derived allergens of Tyrophagus putrescentiae reared on dried dog food and evidence of the strong nutritional interaction between the mite and Bacillus cereus producing protease bacillolysins and exo-chitinases
Erban T, et al.
Frontiers in Physiology, 7, 53-53 (2016)
L Stefanis et al.
Journal of neurochemistry, 69(4), 1425-1437 (1997-11-05)
Rat pheochromocytoma (PC12) cells and sympathetic neurons undergo apoptotic cell death upon withdrawal of trophic support. We have shown previously that selective cysteine aspartase (caspase) inhibitors protect PC12 cells and sympathetic neurons from such death, and that the caspase Nedd-2
Swaantje Peters et al.
Cytokine, 40(2), 144-150 (2007-10-26)
Vascular permeability is important at many sites, but particularly so in diabetic retinopathy where macular oedema is the major cause of blindness. Angiopoietin-2 (Ang-2) and vascular endothelial growth factor (VEGF) are important factors involved in neovascularization and vascular leakage, but
P Kwo et al.
The American journal of physiology, 268(4 Pt 1), G613-G621 (1995-04-01)
Glycodeoxycholate (GDC) induces apoptosis in hepatocytes by a mechanism associated with DNA cleavage by endonucleases. In many models of apoptosis, proteolysis is required prior to DNA cleavage. Our aims were to determine if enhanced proteolysis is a mechanism causing GDC-mediated
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique