T6451

Anti-Twist1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-Twist homolog 1 (acrocephalosyndactyly 3, Saethre-Chotzen syndrome), Anti-Twist related protein 1 (H-twist)
• Numéro MDL: MFCD08705531
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~26 kDa
• Espèces réactives: rat, human, mouse
• Conditionnement: antibody small pack of 25 μL
• Concentration: ~1 mg/mL
• Technique(s): indirect immunofluorescence: 1-2 μg/mL using human osteosarcoma MG-63 cells; western blot (chemiluminescent): 0.5-1 μg/mL using human 293T cells expressing recombinant human Twist1
• Numéro d'accès UniProt: Q15672
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... TWIST1(7291) ; mouse ... Twist1(22160) ; rat ... Twist1(85489)

Description

Description générale

Twist is encoded by the gene mapped to human chromosome 7p21.1. The encoded protein is a basic helix-loop-helix (bHLH) transcription factor. Twist 1 belongs to the Twist gene family and is mainly expressed in the suture mesenchyme.

Spécificité

Anti-Twist1 antibody recognizes human Twist1. Detection of the Twist1 band by immunoblotting is specifically inhibited with the immunizing peptide.

Immunogène

synthetic peptide corresponding to amino acids 12-27 of human Twist1 with a C-terminal added cysteine, conjugated to KLH. The corresponding peptide sequence is identical in both rat and mouse.

Application

Anti-Twist1 antibody is suitable for use in detecting Twist1 via in immunochemical applications such as immunoblotting (~26 kDa) and immunofluorescence.
Immunoblotting: a working antibody concentration of 0.5-1 mg/mL is recommended using extracts of human 293T cells expressing recombinant human Twist1 and a chemiluminescent detection reagent.

Anti-Twist1 antibody produced in rabbit has been used in:

• immunohistochemistry
• immunofluorescence
• immunoblotting
• chromatin immunoprecipitation (ChIP)

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)

Actions biochimiques/physiologiques

Twist is required for mesoderm formation. Twist 1 controls embryonic morphogenesis. It also plays a key role in metastasis by inducing an epithelial-mesenchymal transition (EMT). During an EMT, Twist stimulates the expression of mesenchymal markers, such as fibronectin and N-cadherin. In addition, it also blocks E-cadherin expression inhibiting cell-cell adhesion and therefore inducing infiltrative tumor growth in several human carcinomas. Twist1 is required in head mesenchyme for cranial neural tube morphogenesis in mice. Twist1 is involved in osteoblast differentiation and maturation. Increased expression of Twist 1 in breast cancer cells induce angiogenesis and leads to chromosomal instability. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome, an autosomal dominant defect characterized by minor skull and limb anomalies.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable