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T5701

Sigma-Aldrich

Anti-TSG101 antibody produced in rabbit

~2 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-Tumor Suspectibility Gene 101

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About This Item

Numéro MDL:
MFCD08705528
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen 46 kDa

Espèces réactives

rat, mouse, human

Concentration

~2 mg/mL

Technique(s)

western blot: 0.5-1 μg/mL using cell lysates of rat fibroblast Rat-1 and mouse fibroblast NIH3T3 cells
western blot: 2-4 μg/mL using cell lysates of human A431 cells

Numéro d'accès UniProt

Q99816

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... TSG101(7251)
mouse ... Tsg101(22088)
rat ... Tsg101(292925)

Description générale

Tumor susceptibility 101 (TSG101) is encoded by the gene mapped to human chromosome 11p15.1–15.2. The encoded protein is a member of catalytically inactive ubiquitin E2 variant (UEV) family. Tsg101 is characterized with an ubiquitin E2 variant (UEV) domain in its N-terminal end involved in HIV-1 budding and the vacuolar protein sorting (VPS) pathway.

Immunogène

syntheitic peptide corresponding to amino acids 254-272 located near the C-terminus of human TSG101, conjugated to KLH. This sequence is identical in rat and mouse TSG101.

Application

Anti-TSG101 antibody produced in rabbit has been used in western blot analysis.

Actions biochimiques/physiologiques

Tumor susceptibility 101 (TSG101) is implicated in various cellular function such as, ubiquitination, transcriptional regulation, endosomal trafficking and cell proliferation. Mutation in the gene leads to various human cancers.Tsg101 plays a crucial role in cell growth, cell survival, and normal function of embryonic and adult tissues. TSG101 facilitates infectious human immunodeficiency virus (HIV-1) budding by linking the p6 late domain to vacuolar protein sorting machinery. Tsg101 is a vital constituent of the endosomal sorting complex required for transport (ESCRT) machinery, that helps in synthesis of multi-vesicular bodies, mainly the formation and scission of the intraluminal vesicles, and is thus, crucial for the degradation and recycling of receptors localized on plasma membrane. Tsg101 expression is controlled by an intrinsic autoregulatory mechanism and by ubiquitination catalyzed by various ubiquitin ligases.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium axide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Owen Pornillos et al.
The EMBO journal, 21(10), 2397-2406 (2002-05-15)
Human Tsg101 plays key roles in HIV budding and in cellular vacuolar protein sorting (VPS). In performing these functions, Tsg101 binds both ubiquitin (Ub) and the PTAP tetrapeptide 'late domain' motif located within the viral Gag protein. These interactions are
Comparison of isolation methods of exosomes and exosomal RNA from cell culture medium and serum
Tang YT, et al.
International Journal of Molecular Medicine, 40(3), 834-844 (2017)
Yue-Ting Tang et al.
International journal of molecular medicine, 40(3), 834-844 (2017-07-25)
Exosomes are cell-derived vesicles and are abundant in biological fluids; they contain RNA molecules which may serve as potential diagnostic biomarkers in 'precision medicine'. To promote the clinical application of exosomal RNA (exoRNA), many isolation methods must be compared and validated.
Kay-Uwe Wagner et al.
Molecular and cellular biology, 23(1), 150-162 (2002-12-17)
Tumor susceptibility gene 101 (Tsg101) was identified in a random mutagenesis screen for potential tumor suppressors in NIH 3T3 cells. Altered transcripts of this gene have been detected in sporadic breast cancers and many other human malignancies. However, the involvement
James V McCann et al.
Scientific reports, 9(1), 16743-16743 (2019-11-16)
Extracellular vesicles (EVs) play important roles in tumor progression by altering immune surveillance, promoting vascular dysfunction, and priming distant sites for organotropic metastases. The miRNA expression patterns in circulating EVs are important diagnostic tools in cancer. However, multiple cell types
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