T3405

3,3′,5,5′-Tetramethylbenzidine dihydrochloride

chromogenic, tablet

• Synonyme(s) : 4,4′-Diamino-3,3′,5,5′-tetramethylbiphenyl dihydrochloride
• Formule empirique (notation de Hill): C₁₆H₂₀N₂ · 2HCl
• Numéro CAS: 64285-73-0
• Poids moléculaire : 313.27
• Numéro CE : 264-769-6
• Numéro MDL: MFCD00012961
• Code UNSPSC : 12352204
• ID de substance PubChem : 24900140
• Nomenclature NACRES : NA.83

Propriétés

• Nom du produit: 3,3′,5,5′-Tetramethylbenzidine dihydrochloride, tablet, 1 mg substrate per tablet
• Niveau de qualité: 200
• Forme: tablet
• Pf: ≥300 °C
• Température de stockage: 2-8°C
• Chaîne SMILES: Cl[H].Cl[H].Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
• InChI: 1S/C16H20N2.2ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;/h5-8H,17-18H2,1-4H3;2*1H
• Clé InChI: NYNRGZULARUZCC-UHFFFAOYSA-N

Description

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H₂SO₄ (resulting in a yellow color), and read at 450 nm.

3,3′,5,5′-Tetramethylbenzidine dihydrochloride has been used as a substrate for the

• anti-mouse IgG (? specific) horseradish peroxidase-conjugated antibody in the ELISA of mice sera[1]
• anti-mouse peroxidase antibody in the protein tyrosine phosphatase assay of naive and effector T cell lysate[2]
• in enzymatic hydroperoxide (EH) assay and in prooxidants–antioxidants balance (PAB) assay of serum samples from diabetic patients[3]

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)