STS0033

Agarose tablets

Low EEO, For molecular biology (200 x 500mg tablets)

• Synonyme(s) : 3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose LE, Agarose tablets
• Numéro CAS: 9012-36-6
• Numéro MDL: MFCD00081294
• Code UNSPSC : 41105317
• Nomenclature NACRES : NB.21

Propriétés

• Source biologique: algae (marine)
• Qualité: for molecular biology
• Forme: tablet
• Impuretés: ≤10.0% moisture content
• Couleur: white
• EEO: 0.09-0.13 (-Mr)
• Température de transition: transition temp 34.5-37.5 °C
• Résistance du gel: ≥1200 g/cm² (1% gel)
• Traces d'anions: sulfate (SO₄^2-): ≤0.15%
• Adéquation: suitable for electrophoresis; suitable for molecular biology
• Activité étrangère: DNase, RNase, none detected
• InChI: 1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
• Clé InChI: MJQHZNBUODTQTK-WKGBVCLCSA-N

Description

Description générale

Agarose is commonly used in research for gel electrophoresis to separate DNA and RNA fragments. It is a natural polysaccharide isolated and purified from red algae. It has a linear structure consisting of alternating D-galactose and 3,6-anhydro-L-galactose units. When dissolved in solution, upon cooling, it forms a solid yet porous gel to use as a medium to separate nucleic acid fragments by size. When an electrical current is applied the charged molecules migrate through the gel.

Application

Agarose tablets can be used in research applications for:

• Separation and analysis of nucleic acids by agarose gel electrophoresis, Northern Blot, or Southern Blot
• Single Cell Gel Electrophoresis (SCGE), also known as comet assays
• Hydrocolloid gels to study the structural influence of gels on the release of carbohydrates
• Ouchterlony double immunodiffusion plates used to quantify antibodies and antigens
• Radial immunodiffusion (RID)

Caractéristiques et avantages

• Qualified for molecular biology applications
• Free of endonucleases: DNase and RNase
• Convenient, quick and easy to use
• Low EEO and gelling temperature for routine analysis
• High gel strength 1,200 g/cm2 (1% gel)
• Separates DNA & RNA fragments 100bp - 23kb in 2% - 0.5% agarose gel, respectively.
• Northern or Southern blotting, preferred for fragment sizes >1kb

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable