SRP0212

PTP1B (1-321)/PTPN1 Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Synonyme(s) : PTPN1, Protein tyrosine phosphatase, non-receptor type 1
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in E. coli
• Pureté: ≥90% (SDS-PAGE)
• Forme: aqueous solution
• Poids mol.: 63.3 kDa
• Conditionnement: pkg of 20 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Concentration: 0.84 mg/mL
• Numéro d'accès NCBI: M31724
• Numéro d'accès UniProt: P18031
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... PTPN1(5770)

Description

Description générale

Protein tyrosine phosphatase, catalytic domain, (amino acids 1-321), (Gene bank accession no. M31724) with N-terminal GST tag, MW = 63.3 kDa, expressed in an E. coli expression system.

Protein tyrosine phosphatase, non-receptor type 1 (PTPN1), which is also known as protein-tyrosine phosphatase 1B (PTP1B), is a tyrosine phosphatase. It is localized at the cytoplasmic side of the endoplasmic reticulum. The gene encoding PTPN1 is localized on human chromosome 20q13.13.

Application

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

Actions biochimiques/physiologiques

Protein tyrosine phosphatase, non-receptor type 1 (PTPN1) or protein-tyrosine phosphatase 1B (PTP1B) dephosphorylates the epidermal growth factor receptor. It acts as a modulator of insulin-like growth factor receptor (IGFR) and MET-mediated signaling responses. It may be useful as a drug target in type 2 diabetes and obesity. The protein has been shown to enhance tumor progression in various cancers such as breast, colon and prostate. It acts a tumor suppressor in other cancers (lymphomas and esophageal cancers).

Définition de l'unité

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Forme physique

Formulated in 25 Mm Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable