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SAE0045

Sigma-Aldrich

HRV-3C Protease.

N-Terminal His tagged recombinant protein, aqueous solution, 0.8-1.2 mg/mL

Synonyme(s) :

Human Rhinovirus 3C Protease, Levlfqgp site protease, PreScission Protease

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About This Item

Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Source biologique

human (human Rhinovirus Type 14)

Niveau de qualité

200

Pureté

≥90% (SDS-PAGE)

Forme

aqueous solution

Activité spécifique

≥5000 U/mg

Poids mol.

21 kDa

Concentration

0.8-1.2 mg/mL

Technique(s)

protein purification: suitable

Adéquation

suitable for protein modification

Application(s)

life science and biopharma

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

HRV-3C protease from human rhinovirus type 14 is a protease that specifically cleaves within an eight-residue recognition sequence.
Proteolytic cleavage occurs between the Gln and Gly residues.
HRV-3C protease is a useful tool to cleave recombinant proteins that are expressed as a fusion protein with this sequence, between the carrier domain and the protein of interest.
This recombinant version contains a six-histidine tag and can be easily removed by IMAC chromatography.

Application

HRV-3C Protease has been used to remove the GST tag from the DsCystatin protein.

Définition de l'unité

One unit of HRV-3C Protease is defined as the amount of enzyme needed to digest 1nmole of the substrate peptide per hour (H-Glu-Ala-Leu-Phe-Gln-pNA) at 0 °C, in a reaction buffer consisting of 25 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM EDTA, and 1 mM DTT.

Forme physique

Supplied in a solution containing 50 mM Tris HCl (pH 7.5), 0.15 M NaCl, 1 mM TCEP and 50% (V/V) glycerol.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

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