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SAB4200670

Sigma-Aldrich

Anti-Myosin (Skeletal, Slow) antibody, Mouse monoclonal

clone NOQ7.5.4D, purified from hybridoma cell culture

Synonyme(s) :

Monoclonal Anti-Myosin (Skeletal, Slow) antibody produced in mouse, CMD1S, CMH1, MPD1, MYHCB, SPMD, SPMM

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

200

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

NOQ7.5.4D, monoclonal

Forme

buffered aqueous solution

Espèces réactives

sheep, goat, chicken, feline, guinea pig, pig, rabbit, hamster, canine, rat, human, mouse, bovine

Conditionnement

antibody small pack of 25 μL

Concentration

~1 mg/mL

Technique(s)

immunoblotting: 1.25-2.5 μg/mL using Rat skeletal muscle lysate.
immunohistochemistry: 2.5-5 μg/mL using pronase digested, formalin-fixed, paraffin-embedded rabbit tongue/leg sections and Biotin/ExtrAvidin®-Peroxidase staining system.

Isotype

IgG1

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... MYH7(4625)

Description générale

Monoclonal Anti-Myosin (Skeletal, Slow) (mouse IgG1 isotype) is derived from the NOQ7.5.4D hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Myosin is a hexameric protein containing two heavy chain subunits and four light chain subunits. It is a major protein component of heart and skeletal muscles. Muscle myosin heavy chain (MHC) different isoforms are expressed depending on the physiological function of the muscle: skeletal, cardiac, smooth and non-muscle.

Immunogène

Myosin, purified from myofibrils isolated from human skeletal muscle,

Application

Monoclonal Anti-Myosin (Skeletal, Slow) antibody produced in mouse has been used in immunobloting, immunohistology, Solid-phase radioimmunoassay (RIA), enzyme linked immunosorbent assay (ELISA) and immunoelectronmicroscopy.

Actions biochimiques/physiologiques

Myosin are actin-dependent motors. Mutations in myosins genes are associated with myopathies, blindness and hearing loss. They are involved in muscle contraction, vision, hearing, cell movements and cell invasion.
Myosin is the element responsible for energy transduction and force development in heart and skeletal muscles. Myosin is known to interact with actin in muscle and in nonmuscle cells. Slow myosin, also known as β-myosin heavy chain (β-MHC) is expressed during embryogenesis as part of the cardiac myogenic program under the control of NK-2 homeobox protein-5 (NKX-2.5), myocyte enhancer factor-2C (MEF-2C), and GATA-4/5/6.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Informations légales

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Bernardo J Foth et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(10), 3681-3686 (2006-03-01)
Myosins are eukaryotic actin-dependent molecular motors important for a broad range of functions like muscle contraction, vision, hearing, cell motility, and host cell invasion of apicomplexan parasites. Myosin heavy chains consist of distinct head, neck, and tail domains and have
Jelle C B C de Jong et al.
Biology of sex differences, 14(1), 45-45 (2023-07-11)
Physical weakness is a key component of frailty, and is highly prevalent in older adults. While females have a higher prevalence and earlier onset, sex differences in the development of frailty-related physical weakness are hardly studied. Therefore, we investigated the
Heavy and light roles: myosin in the morphogenesis of the heart
England J and Loughna S
Cellular and Molecular Life Sciences, 70(7), 1221-1239 (2013)
Jelle C B C de Jong et al.
GeroScience, 46(3), 3341-3360 (2024-01-24)
Muscle-aging drives sarcopenia and is a major public health issue. Mice are frequently used as a model for human muscle-aging, however, research investigating their translational value is limited. In addition, mechanisms underlying muscle-aging may have sex-specific features in humans, but
Caloric Restriction Combined with Immobilization as Translational Model for Sarcopenia Expressing Key-Pathways of Human Pathology.
de Jong, et al.
Aging and disease, 14, 937-957 (2023)
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