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SAB4200119

Sigma-Aldrich

Monoclonal Anti-FLAG-Peroxidase antibody produced in rat

2-4 mg/mL, clone 6F7, purified immunoglobulin

Synonyme(s) :

Anti-ddddk, Anti-dykddddk

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.32

Source biologique

rat

Niveau de qualité

200

Conjugué

peroxidase conjugate

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

6F7, monoclonal

Forme

buffered aqueous solution

Espèces réactives

all

Concentration

2-4 mg/mL

Technique(s)

western blot: 1:1,000-1:2,000 using extracts of transfected cells expressing C-terminal FLAG-tagged fusion protein

Isotype

IgG1

Séquence immunogène

DYKDDDDK

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

Monoclonal Anti-FLAG-Peroxidase is a purified immunoglobulin fraction of monoclonal Anti-FLAG (rat IgG1 isotype) isolated from culture supernatant of the 6F7 hybridoma cells grown in a bioreactor, conjugated to horseradish peroxidase (HRP). The hybridoma 6F7 was produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with the FLAG peptide.
The product recognizes N-terminal, C-terminal, and internal FLAG-tagged fusion proteins. It is especially recommended for identifying C-terminal FLAG-tagged fusion proteins.

Immunogène

purified immunoglobulin fraction of monoclonal Anti-FLAG (rat IgG1 isotype) isolated from culture supernatant of the 6F7 hybridoma cells grown in a bioreactor, conjugated to horseradish peroxidase (HRP).

Application

Learn more product details in our FLAG® applications portal.
Monoclonal Anti-FLAG-Peroxidase, recognizes N-terminal, C-terminal and internal FLAG-tagged fusion proteins. The product is especially recommended for identifying C-terminal FLAG-tagged fusion proteins. The product can be used for immunoblotting.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% merthiolate.

Informations légales

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Jente Stouthamer et al.
Methods in molecular biology (Clifton, N.J.), 2690, 193-204 (2023-07-14)
Interactions between extracellular domains (ECDs) are crucial for many physiological processes in the cell, most importantly perception of its environment. However, studying these often-transient interactions can be challenging. Here we describe a method that allows for in vitro detection of
Jasjot Singh et al.
Nature communications, 13(1), 6212-6212 (2022-10-21)
Lysosomes are well-established as the main cellular organelles for the degradation of macromolecules and emerging as regulatory centers of metabolism. They are of crucial importance for cellular homeostasis, which is exemplified by a plethora of disorders related to alterations in
Claudia Isabelle Keller Valsecchi et al.
Nature, 589(7840), 137-142 (2020-11-20)
Confinement of the X chromosome to a territory for dosage compensation is a prime example of how subnuclear compartmentalization is used to regulate transcription at the megabase scale. In Drosophila melanogaster, two sex-specific non-coding RNAs (roX1 and roX2) are transcribed
DongHyuk Lee et al.
Nature communications, 11(1), 1838-1838 (2020-04-17)
Production of reactive oxygen species (ROS) is critical for successful activation of immune responses against pathogen infection. The plant NADPH oxidase RBOHD is a primary player in ROS production during innate immunity. However, how RBOHD is negatively regulated remains elusive.
Meidi Gu et al.
Nature immunology, 22(2), 193-204 (2021-01-06)
Metabolic reprograming toward aerobic glycolysis is a pivotal mechanism shaping immune responses. Here we show that deficiency in NF-κB-inducing kinase (NIK) impairs glycolysis induction, rendering CD8+ effector T cells hypofunctional in the tumor microenvironment. Conversely, ectopic expression of NIK promotes
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