S9811
S-Gal®
reagent for selection of recombinant bacterial clones
Synonyme(s) :
3,4-Cyclohexenoesculetin β-D-galactopyranoside
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About This Item
Formule empirique (notation de Hill):
C19H22O9
Numéro CAS:
Poids moléculaire :
394.37
Code UNSPSC :
12352200
ID de substance PubChem :
Nomenclature NACRES :
NA.85
Produits recommandés
Qualité
for molecular biology
Niveau de qualité
Stérilité
non-sterile
Essai
≥95% (HPLC)
Forme
powder
Solubilité
DMF: 50 mg/mL
Adéquation
suitable for β-galactosidase test
Température de stockage
room temp
Chaîne SMILES
OC[C@H]1O[C@@H](Oc2cc3OC(=O)C4=C(CCCC4)c3cc2O)[C@H](O)[C@@H](O)[C@H]1O
InChI
1S/C19H22O9/c20-7-14-15(22)16(23)17(24)19(28-14)27-13-6-12-10(5-11(13)21)8-3-1-2-4-9(8)18(25)26-12/h5-6,14-17,19-24H,1-4,7H2/t14-,15+,16+,17-,19-/m1/s1
Clé InChI
HDJJDRXZHJRVGA-DIKXUDHVSA-N
Description générale
S-Gal® is an autoclavable, chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. S-Gal® is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Application
Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. S-Gal® is autoclavable and can be added to bacterial broth containing agar prior to autoclaving.
S-Gal is a patented autoclavable chromogenic substrate for β-galactosidase that is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Caractéristiques et avantages
- More intense color contrast than X-gal
- Excellent for use in automated colony counters
- Autoclavable for easiest use
- No need to make stock solutions
When S-Gal is cleaved by β-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies. S-Gal is autoclavable and can be added to your medium of choice prior to autoclaving. S-Gal is not light sensitive and does not require any protection from light sources.
Autres remarques
Autoclavable, microwavable growth medium complete with S-Gal and IPTG.
The ferric or Fe3+ ion is required for color development and must be added to any S-Gal®
formulation. A medium prepared with S-Gal® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation.
formulation. A medium prepared with S-Gal® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation.
Principe
When S-Gal® is cleaved by ß-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal® and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies.
Reconstitution
Stock solutions of S-Gal® can be made by dissolving 50mg/ml in dimethyl formamide (DMF) and storing at -20C. Add S-Gal® (300 mg/L from stock solution ) and Ferric Ammonium Citrate (500mg/L) to agar media prior to autoclaving.
Informations légales
S-GAL is a registered trademark of Merck KGaA, Darmstadt, Germany
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Organes cibles
Respiratory system
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
dust mask type N95 (US), Eyeshields, Gloves
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