S7571

Superoxide Dismutase from bovine erythrocytes

lyophilized powder, ≥3,000 units/mg protein, Protein ≥95 % by biuret

• Synonyme(s) : CU/ZN-SOD, Superoxide Dismutase 1 bovine, cytocuprein, erythrocuprein, hemocuprein, SOD, Superoxide: superoxide oxidoreductase
• Numéro CAS: 9054-89-1
• Numéro de classification (Commission des enzymes): 1.15.1.1 (BRENDA, IUBMB)
• Numéro CE : 232-943-0
• Numéro MDL: MFCD00132404
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: bovine
• Niveau de qualité: 300
• Forme: lyophilized powder
• Activité spécifique: ≥3,000 units/mg protein
• Poids mol.: 32.5 kDa
• Composition: Protein, ≥95% biuret
• Conditions de stockage: (Store under nitrogen.; Tightly closed. Dry.)
• Caractéristiques du produit alternatif plus écologique: Atom Economy; Design for Energy Efficiency; Use of Renewable Feedstocks; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• Technique(s): immunoblotting: suitable; inhibition assay: suitable
• Couleur: blue-green
• pl: 4.95
• Solubilité: water: 20 mg/mL; aqueous buffer, pH 7.5: soluble
• Numéro d'accès UniProt: P00442; P41976
• Application(s): diagnostic assay manufacturing
• Autre catégorie plus écologique: , Re-engineered
• Température de stockage: −20°C
• Informations sur le gène: cow ... SOD1(281495) , SOD2(281496)

Description

Description générale

Research area: Cell Signaling

Superoxide dismutase (SOD) is a redox-active metalloenzyme expressed in both aerobic and anaerobic living organisms. Bovine superoxide dismutase or CuZn SOD is a homodimer with each subunit containing one zinc and one copper ion.

Application

Superoxide dismutase from bovine erythrocytes has been used:

• in a study to assess a kinetic model of radiation-induced inactivation of superoxide dismutase in nitrous oxide-saturated solutions
• in a study to investigate the possible participation of superoxide anion in the intestinal tryptophan 2,3-dioxygenase reaction
• to investigate its effect on the hemolysis rate of human RBCs and hemoglobin-nitric oxide complex (HbNO) stability in human erythrocytes
• in combination with catalase to study its effect on cell differentiation in vitro
• to quantify superoxide levels and study their effect on reactivity in mouse pulmonary arteries through chemiluminescence and cytochrome C reduction methods

Actions biochimiques/physiologiques

Superoxide Dismutase from bovine erythrocytes catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. It serves as an antioxidant and plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

Définition de l'unité

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 ml reaction volume. Xanthine oxidase concentration should produce an initial ΔA₅₅₀ of 0.025 ± 0.005 per min.

Forme physique

Lyophilized powder, essentially salt-free

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)