S5171
Human Fibronectin
formulated with recombinant human fibronectin fragment III-C expressed in E. coli and fibronectin from human plasma, liquid, 2 mg/mL, suitable for cell culture
Synonyme(s) :
sFN
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About This Item
Produits recommandés
product name
Superfibronectin from human plasma, solution, BioReagent, suitable for cell culture
Source biologique
human
Niveau de qualité
Produit recombinant
expressed in E. coli
Gamme de produits
BioReagent
Forme
solution
Poids mol.
~235-270 kDa
Conditionnement
pkg of 5 mg
Technique(s)
cell culture | mammalian: suitable
Couverture de surface
1 μg/cm2
Numéro d'accès UniProt
Conditions d'expédition
ambient
Température de stockage
2-8°C
Informations sur le gène
human ... FN1(2335)
Application
Superfibronectin from human plasma dramatically enhances the adhesive properties of fibronectin and suppresses cell migration. Improved results have been shown CHO cells, IMR-90 fibroblasts, T47D breast carcinoma cells, and UCLA-P lung carcinoma cells. Additional applications include epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, and endothelial cells. It is recommended for use as a cell culture substratum at 1 μg/cm2.
Composants
Superfibronectin is formulated with recombinant, human fibronectin fragment III-C expressed in E. coli and fibronectin from human plasma. The recombinant fibronectin includes a new region, type III, cloned from human placenta cDNA, that is newly reported to participate in matrix formation by inducing spontaneous disulfide crosslinking of fibronectin molecules into multimers. The product resembles the in vivo matrix form of fibronectin.
Attention
Superfibronectin should be stored at 2-8°C and diluted immediately prior to use. Do not freeze this product.
Notes préparatoires
As supplied, this product is a 2 mg protein/mL solution in 0.05 M Tris buffered saline. Before coating a 96 well plate, it is recommended to dilute t he product with sterile PBS to a concentration of 5 μg/mL and add 100 μl/well. These plates should then be incubated for 2 hours at 37°C and washed twice with 100 μl of PBS. The source material has been tested for HBsAg and HIV antibodies.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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