S3917
Single-strand Binding Protein from Escherichia coli
>95% (SDS-PAGE), for molecular biology, Single strand binding protein to enhance PCR specificity
Synonyme(s) :
SSB
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About This Item
Produits recommandés
Qualité
for molecular biology
Niveau de qualité
Pureté
>95% (SDS-PAGE)
Forme
liquid
Couleur
colorless
Activité étrangère
DNase and RNase, none detected
Conditions d'expédition
wet ice
Température de stockage
−20°C
Description générale
Single-strand Binding Protein is a homotetramer, found in E. coli during the event of DNA metabolism.
Application
Binds with high specificity to single-stranded DNA. Can be useful in enhancing the specificity of PCR and in enabling the sequencing of problematic DNA templates.
Single-strand Binding Protein from Escherichia coli has been used in in vitro RNA/Single-Strand Binding Protein (SSB) complex formation assays and in protein purification to determine integrity and functionality of the integron attC sites.
Actions biochimiques/physiologiques
Single-strand Binding Protein (SSBP) exhibits high affinity towards single stranded DNA important for DNA metabolic processes. Single-stranded DNA-binding protein hSSB1 is critical for genomic stability and assists the delivery of primers during the formation of lagging standard. It also prevents the formation of inhibitory secondary structures in the exposed lagging-strand of DNA during replication. SSBP mediates the recruitment of other proteins to their sites of function on DNA.
Forme physique
Solution in 20 mM Tris-HCl, pH 8.0, 0.5 M NaCl, 0.1 mM EDTA, 0.1 mM DTT, 50% glycerol
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
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J Kieleczawa et al.
Science (New York, N.Y.), 258(5089), 1787-1791 (1992-12-11)
When template DNA is saturated with a single-stranded DNA binding protein (SSB), strings of three or four contiguous hexanucleotides (hexamers) can cooperate through base-stacking interactions to prime DNA synthesis specifically from the 3' end of the string. Under the same
Binding Specificity of E. coli SSB protein for the ? subunit of DNA pol III Holoenzyme and PriA helicase
Alexander GK, et al.
Biochemistry, 49(17), 3555-3556 (2010)
Minimizing deletion mutagenesis artifact during Taq DNA polymerase PCR by E. coli SSB.
Q Chou
Nucleic acids research, 20(16), 4371-4371 (1992-08-25)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 87, 5-5 (1989)
Structure of the SSB--DNA polymerase III interface and its role in DNA replication
Marceau AH, et al.
The Embo Journal, 30(20), 4236-4247 (2011)
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