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S2519

Sigma-Aldrich

Anti-Soy Protein antibody produced in rabbit

fractionated antiserum, buffered aqueous solution

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

rabbit

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

fractionated antiserum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Espèces réactives

soy

Technique(s)

dot blot: 1:500
indirect ELISA: 1:5,000

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

Some cross-reaction is observed with extracts from wheat flour and ovalbumin; no cross-reaction is observed with extracts from maize, rice, potato flour, casein, or meat.
Soy protein is obtained from soybeans. This legume is free of cholesterol and is low in saturated fat. It is mainly used as a replacement for animal proteins in an individual′s diet. This vegetable food has all eight essential amino acids. Soy protein can be used as meat extenders or analogues.

Immunogène

renatured soy protein extracted from soy acetone powder with hot urea.

Application

Anti-Soy Protein antibody produced in rabbit has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • supershift analysis
  • fluorescent imaging
  • immunostaining to probe polyvinylene diflouride (PVDF)-bound proteins for cross-reactivity

Anti-Soy Protein antibody produced in rabbit was used for soy quantification in natural rubber latex glove extracts by ELISA at 1:2000 dilution. It was used as a control in supershift analysis of growth-hormone-induced protein binding to lactogenic hormone-responsive region.

Actions biochimiques/physiologiques

Soy protein is a rich source of isoflavones that act as estrogen analogs. They exert both estrogenic and anti-estrogenic effects depending on the tissue in which they act. Isoflavones effect the lipid and bone metabolism and may act as a potential alternative to estrogen in post-menopausal women.

Description de la cible

Soy protein in a variety of forms is the basis of many low cholesterol foods and may also be incorporated in animal meat products.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Priyae Brath Gautam et al.
Journal of food science and technology, 54(13), 4213-4219 (2017-12-01)
A lateral flow based detection method for ascertaining the presence of soymilk in whole bovine milk has been described. The method uses commercially available rabbit anti-soy protein antibodies conjugated to gold nanoparticles (AuNPs) wherein soymilk protein in adulterated milk and
Soy protein
Montgomery KS
The Journal of perinatal education, 12(3), 42-42 (2003)
S M Potter et al.
The American journal of clinical nutrition, 68(6 Suppl), 1375S-1379S (1998-12-16)
The effects of soy protein (40 g/d) containing moderate and higher concentrations of isoflavones on blood lipid profiles, mononuclear cell LDL receptor messenger RNA, and bone mineral density and content were investigated in 66 free-living, hypercholesterolemic, postmenopausal women during a
D L Alekel et al.
The American journal of clinical nutrition, 72(3), 844-852 (2000-09-01)
No published studies have directly examined the effect of soy protein with isoflavones on bone or bone turnover in perimenopausal women. Our objective was to determine the effects of 24 wk of consumption of soy protein isolate with isoflavones (80.4
Activation of the signal transducers and activators of transcription signaling pathway by growth hormone (GH) in skin fibroblasts from normal and GH binding protein-positive Laron Syndrome children
Freeth JS, et al.
Endocrinology, 139(1), 20-28 (1998)

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